202280. lajstromszámú szabadalom • Eljárás funkcionális humán urokináz fehérjék előállítására
1 61 121 181 241 301 361 421 481 541 601 661 721 HU 202280 B Int CP c 12 N 15/58 C 12 N 15/70 C 12 N 15/85 PstI Mnll Mnll MboII CTGCAGGGGGGGGGGAGATGGAAAAAAGCCCTCCTCTCCTCCAGAAGAATTAAAATTTCA GACGTCCCCCCCCCCTCTACCTTTTTTCGGGAGGAGAGGAGGTCTTCTTAATTTTAAAGT Mill BalIHaeIIIHinfIMnlISau96 EcoRI T GTGTGGCCAAAAGACTCTGAGGCCCCGCTTTAAGATTATTGGGGGAGAATTCACCACCAT CACACCGGTTTTCTGAGACTCCGGGGCGAAATTCTAATAACCCCCTCTTAAGTGGTGGTA Hael DdelHaelII Hphl aql BstNI Fnu4HI Mill Hpall Hphl CGAGAACCAGCCCTGGTTTGCGGCCATCTACAGGAGGCACCGGGGGGGCTCTGTCACCTA GCTCTTGGTCGGGACCAAACGCCGGTAGATGTCCTCCGTGGCCCCCCCGAGACAGTGGAT EcoRII HaelII Ncil MnlIBbv HphIOpnlHaelI CGTGTGTGGAGGCAGCCTCATCAGCCCTTGCTGGGTGATCAGCGCCACACACTGCTTCAT GCACACACCTCCGTCGGAGTAGTCGGGAACGACCCACTAGTCGCGGTGTGTGACGAAGTA Fnu4HIMnlI BclISau3AHhaI Mnll AccI EcoRII TGATTACCCAAAGAAGGAGGACTACATCGTCTACCTGGGTCGCTCAAGGCTTAACTCCAA ACTAATGGGTTTCTTCCTCCTGATGTAGCAGATGGACCCAGCGAGTTCCGAATTGAGGTT BstNI Mnll Mill Haell CACGCAAGGGGAGAT GAAGTTT GAGGTGGAAAAC CT CATCCTACACAAGGACTACAGCGC GTGCGTTCCCCTCTACTTCAAACTCCACCTTTTGGAGTAGGATGTGTTCCTGATGTCGCG Hhal Hphl MboIIDpnI Mnll TGACACGCTTGCTCACCACAACGACATTGCCTTGCTGAAGATCCGTTCCAAGGAGGGCAG ACTGTGCGAACGAGTGGTGTTGCTGTAACGGAACGACTTCTAGGCAAGGTTCCTCCCGTC XholISau3A MstIBbv Neil Will DpnI GTGTGCGCAGCCATCCCGGACTATACAGACCATCTGCCTGCCCTCGATGTATAACGATCC CACACGCGTCGGTAGGGCCTGATATGTCTGGTAGACGGACGGGAGCTACATATTGCTAGG HhaIFnu4HI Hpall TaqT Säü3Ä Alul DpnI EcoRI CCAGTTTGGCACAAGCTGTGAGATCACTGGCTTTGGAAAAGAGAATTCTACCGACTATCT GGTCAAACCGTGTTCGACACTCTAGTGACCGAAACCTTTTCTCTTAAGATGGCTGATAGA Sau3A HpalIFnu4HIPvuII Alul Hpall Fnu CTATCCGGAGCAGCTGAAAATGACTGTTGTGAAGCTGATTTCCCACCGGGAGTGTCAGCA GATAGGCCTCGTCGACTTTTACTGACAACACTTCGACTAAAGGGTGGCCCTCACAGTCGT BbvAluI Ncil Bbv 4HI Hphl Fnu4HI GCCCCACTACTACGGCTCTGAAGTCACCACCAAAATGCTATGTGCTGCTGACCCCCAATG CGGGGT GAT GATGCCGAGACTT CAGT GGTGGTTTT ACGAT ACACGACGACTGGGGGTT AC Bbv HinfI BstNI HinfI AvalIMilI Mnll Haell GAAAACAGATTCCTGCCAGGGAGACTCAGGGGGACCCCTCGTCTGTTCCCTCCAAGGCCG CTTTTGTCTAAGGACGGTCCCTCTGAGTCCCCCTGGGGAGCAGACAAGGGAGGTTCCGGC EcoRII Ddel Sau96 Fnu4 I Alul Sau96 BstNI CATGACT TTGACT GGAATT GT GAGCTGGGGCCüT GGAT GTGCCCTGAAGGACAAGCCAGG GTACTGAAACTGACCTTAACACTCGACCCCGGCACCTACACGGGACTTCCTGTTCGGTCC Hl Hael II EcoRI 1 A. ábra
