202280. lajstromszámú szabadalom • Eljárás funkcionális humán urokináz fehérjék előállítására
781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 HU 202280 B Int Cl5 C 12 N 15/58 C 12 N 15/70 C 12 N 15/85 AcylAccI HinfI EcoRIIBamHISau3A MboII CGTCT ACACGAGA6T CT CACACTTCTT ACCCT GGATCCGCAGTCACACCAAGGAAGAGAA GCAGATGTGCTCTCAGAGTGTGAAGAATGGGACCTAGGCGTCAGTGTGGTTCCTTCTCTT Hgal BstNIXhoIIDpnI HaelBstNIHaelIIMnlIMnlIAvalIEcoRIIMnlI TGGCCTGGCCCTCTGAGGGTCCCCAGGGAGGAAACGGGCACCACCCGCTTTCTTGCTGGT ACCGGACCGGGAGACTCCCAGGGGTCCCTCCTTTGCCCGTGGTGGGCGAAAGAACGACCA HaelIIEcoRIISau96DdeISau96BstNI Hinfl PvuII MboII MboII TGTCATTTTTGCAGTAGAGTCATCTCCATCAGCTGTAAGAAGAGACTGGGAAGATAGGCT ACAGTAAAAACGTCATCTCAGTAGAGGTAGTCGACATTCTTCTCTGACCCTTCTATCCGA Alul EcoRIIHphl Alul Mill CTGCACAGAT GGATTTGCCTGT GGCACCACCAGGGT GAACGACAAT AGCTTT ACCCTCAC GACGTGTCTACCTAAACGGACACCGTGGTGGTCCCACTTGCTGTTATCGAAATGGGAGTG BstNI HaelHaelIIEcoRIIFnu4HI Mi II HaelHaelIIBstNIMilIAvail Hin GGATAGGCCTGGGTGCTGGCTGCCCAGACCCTCTGGCCAGGATGGAGGGGTGGTCCTGAC CCTATCCGGACCCACGACCGACGGGTCTGGGAGACCGGTCCTACCTCCCCACCAGGACTG StuIBstNI Bbv BalIEcoRII Sau96 fi PstI TCAACATGTTACTGACCAGCAACTTGTCTTTTTCTGGACTGAAGCCTGCAGGAGTTAAAA AGTTGTACAATGACTGGTCGTTGAACAGAAAAAGACCTGACTTCGGACGTCCTCAATTTT Taql Alul Hpall AGGGCAGGGCATCTCCTGTGCATGGGCTCGAAGGGAGAGCCAGCTCCCCCGACCGGTGG6 TCCCGTCCCGTAGAGGACACGTACCCGAGCTTCCCTCTCGGTCGAGGGGGCTGGCCACCC Mn IIHaelII XmnI Fnu4HI CATTTGTGAGGCCCATGGTTGAGAAATGAATAATTTCCCAATTAGGAAGTGTAAGCAGCT GTAAACACTCCGGGTACCAACTCTTTACTTATTAAAGGGTTAATCCTTCACATTCGTCGA Sau96NcoI BbvAlu DdelMnlI Mnll Alul Bbv GAGGTCTCTTGAGGGAGCTTAGCCAATGTGGGAGCAGCGGTTTGGGGAGCAGAGACACTA CTCCAGAGAACTCCCTCGAATCGGTTACACCCTCGTCGCCAAACCCCTCGTCTCTGTGAT I Ddel Fnu4HI ACGACTTCAGGGCAGGGCTCTGATATTCCATGAATGTATCAGGAAATATATATGTGTGTG TGCTGAAGTCCCGTCCCGAGACTATAAGGTACTTACATAGTCCTTTATATATACACACAC Alul T AT GTTTG C ACACTTGTTGTGTGGGCTGTGAGT GTAAGTGTGAGTAAGAGCT GGTGTCTG AT ACAAACGT GT GAACAACACACCCGACACT CACATT CACACTCATT CT CGACCACAGAC SfaNI ATTGTTAAGTCTAAATATTTCCTTAAACTGTGTGGACTGTGATGCCACACAGAGTGGTCT T AACAATTCAGATTTAT AAAGGAATTTGACACAC CT GACACT ACGGT GT GTCTCACCAGA Mnll EcoRI ISau9bMil IAval I EcoR TTCTGGAGAGGTTATAGGTCACTCCTGGGGCCTCTTGGGTCCCCCACGTGACAGTGCCTG AAGACCTCTCCAATATCCAGTGAGGACCCCGGAGAACCCAGGGGGTGCACTGTCACGGAC BstNIHaelII Sau96 BstN IB.ábra
