197939. lajstromszámú szabadalom • Eljárás humán növekedési hormont felszabadító faktort meghatározó dezoxi-ribonukleinsav vagy prekurzora előállítására
7 197939 8 8.klón: TGGGAACGCCAGGCGGCTGCCAGAGCAAACACCCAGCCCAGGGCCCCTGGATTTGAGCAGTGCCT 21.klón: AGGGCCCCTGGATTTGAGCAGTGCCT 50 100 CGGAGCAGAGGGATATCTGCCGCATCAGGTGCCACCCCGGGTGAAGG ATG CCA CTC TGG GTG TTC 'CGGAGCAGAGGGATATCTGCCGCATCAGGTGCCACCCCGGGTGAAGG ATG CCA CTC TGG GTG TTC preproGRF-108 Met Pro Leu Trp Val Phe preproGRF-107 150 Met Pro Leu Trp Val Phe TTC TTT GTG ATC CTC ACC CTC AGC AAC AGC TCC CAC TGC TCC CCA CCT CCC CCT TTC TTT GTG ATC CTC ACC CTC AGC AAC AGC TCC CAC TGC TCC CCA CCT CCC CCT Phe Phe Val Ile Leu Thr Leu Ser Asn Ser Ser His Cvs Ser Pro Pro Pro Pro Phe Phe Val Ile Leu Thr Leu Ser Asn Ser Ssx. His Cys Ser Pro Pro Pro Pro 10 20 200 TTG ACC CTC AGG ATG CGG CGG TAT GCA GAT GCC ATC TTC ACC AAC AGC TAC CGG TTG ACC CTC AGG ATG CGG CGG TAT GCA GAT GCC ATC TTC ACC AAC AGC TAC CGG Leu Thr Leu Arg Met Arg Arg Tyr Ala Asp Ala Ile Phe Thr Aon Ser Tyr Arg Leu Thr Leu Arg Met Arg Arg Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg 30 40 250 AAG GTG CTG CG C CAG CTG TCC GCC CGC AAG CTG CTC CAG GAC ATC ATG AGC AGG AAG GTG CTG GGC CAG CTG TCC GCC CGC AAG CTG CTC CAG GAC ATC ATG AGC AGG Ly s Val Leu Gly Gin Let) Ser Ala Arg Lys Leu Leu Gin Asp Ile Met Ser Arg Lys Val Leu Gly Gin Leu Ser Ala Arg Lys Leu Leu Gin Asp Ile Met Ser Arg CAG CAG GGA GAG AGC AAC CAA GAG CGA GGA GCA AGG GCA CGG CTT GGT CGT CAG CAG CAG CGA GAG AGC AAC CAA GAG CGA GGA GCA AGG GCA CGG CTT GGT CGT CAG Gin Gin Gly Glu Ser Asn Gin Glu Arg Gi y Ala Arg Alá Arg Leu Gly Arg Gin Gin Gin Gly Glu Ser Asn Gin Glu Arg Gly Ala Arg Ala Arg Leu Gly Arg Gin 350 HTT~ . 400 GTA GAC AGC ATG TGG GCA GAA CAA AAC CAA ATG GAA TTG GAG AGC ATC CTG GTG GTA GAC AGC ATG TGG GCA GAA CAA AAC CAA ATG GAA TTG GAG AGC ATC CTG GTG Val Asp Ser Met Trp Ala Glu Gin Lys Gin Met Glu Leu Glu Ser Ile Leu Val -Val Asp 80 Ser Met Trp Ala Glu Gin Lys Gin Met Glu Leu Glu Ser Ile Leu Val 90 450 GCC CTG CTG CAG AAG CAC AGC AGG AAC TCC CAG CGATGAAGATTCCTCCTGTGACCCGGG GCC CTG CTG CAG AAG CACAGG AAC TCC CAG GGATGAAGATTCCTCCTGTGACCCGGG Ala Leu Leu Gin Lys His Ser Arg Asr Ser Gin Gly Ala Leu Leu Gin 100 Lys His t Arg Asr 500 Ser Gl n Gly CTACCTGTAGCCAAAATGCAACTGGATCCAGTTAATCCTCTCATTTCTGACCCACTTTTTCCTTTGAAAAT CTACCTGTAGCCAAAATGCAACTGGATCCAGTTAATCCTCTCATTTCTGACCCACTTTTTCCTTTGAAAAT 550 570 ACAATAAAATTCCCCCATÁCCGGTGTGCATTTAAATCTTAAAAAAAAAAAAAAAA ACAATAAAATTCCCCCATACCGGTGTGCATTTAAA "t AAAAAAAAAAAAAAAA o
