Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)
Botany
Micropropagation of blackberry cultivars — perspectives for Republic of Moldova All varieties of plants have been micropropagated on the basic medium MS 100% with the addition of growth regulators BAP, I BA, NAA (tab. 2). In order to make the Polar, Arapaho, Thornfree cultivar root, nutritve media MS 50% liquid with the addition of 30g/l sugar and 15/1 sugar was used (Photo 4).The optimal medium for rootedness process is the 50% MS medium, liquid, added with 30g of sugar, root emergence was observed in the first 10 days after transfer on this medium. In 21 days the plant is well rooted and reaches 4 cm in length, the root system has 5—6 roots with 2- 2.5 cm in length (Photo 5). Photo 5: Rootedness process development MS 50% medium for Arapaho The apical part of the plant is transferred to the culture medium to further induce morphogenetic develpment in the in vitro cultue, while the basal part of 2—3 internodes is being transferred to ex vitro to be naturalized and it develops subsequently to achieve the vegetative and generative phases. Rootedness occurs simultaneously at all cutivars, but the roots vary. It was observed that Chester and Loch Ness develop 5-7 unbranched long shoots, with 0.8 mm diameter. Smoothstem and Reuben shoots are rough and have 1 mm diameter . Evergreen has 6-7 shoots and 0,8- 0,9 mm diameter (Photo 3). Thus, we have established that the optimal, cost-effective and appropriate medium for blackberry varieties’ rootedness is MS 50% (Photo 5). Conclusions 1. Chester, Loch Ness, Thornless evergreen, Smoothstem, Remontana, Polar, Arapaho, Thornfree varieties can be successfully approved in Moldova, and can be used as a donor for plant micropropagation. 2. The elaboration of the sterilization protocol of the plant material taken from the donor-plant is an essential contribution to the initiation stage of in vitro culture. 3. It was elaborated a technology to obtain seedling material for qualitative and homogeneous blackberry cultivars through in vitro cultures, the stages of initiating, microcloning and 15
