Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)

Botany

Mariana LOZINSCHII, Nina CIORCHINÄ, Tatiana CALALB

rootedeness are described. The most appropriate and optimal substrate for the growth of plants is MS 100% and 50% for maintenance and rootedness.

4. The growth and rootedness processes were initiated on a more economic medium, MS 50% where the rootedness is formed in 20 days, and the shoot growth is vigorous and successfully participates in the micropropagation process.

5. Suplimented BAP in the basic medium that has selective action played an important role in the initiation of morphogenesis. Rootedness was stimulate by filling the nutrient medium with IBA and NAA, so the rooting became visible after 10-12 days after cultivation.

REFERENCES

1. Clapa, D., Fira, A, Dumitras, A., Ciorchinä, N., 2011, Inrädäcinarea si aclimatizarea ex vitro in hidrocultura

prin flotatie a unor genotipuri de mur, Revista Botanica, Vol. Ill, Nr.3, Chi§inäu: 133—140.

2. Fira, A., Clapa, D., Vescan, L„ 2012, The Direct ex Vitro Rooting and Acclimation of some Florticultural Species

in Jiffy 7 Pellets, Bulletin SAMVAnimal Sciences and Biotechnologies, 69(1—2)/2012, Print ISSN 1843—5262; Electronic ISSN 1843-536X: 349-350.

3. Gajdosova, A., Ostrolucka, M. G.,. Libiakova, V., Odruskova, E., Simala, D., 2006, Microclonal Propagation

of Vaccinium sp. and Rubus sp. and detection of genetic variability of culture in vitro, Journal of Fruit and Ornamental Plant Research, 14 (1):103-119.

4. Lozinschii, M., Ciorchinä, N., 2014, Growth Regulators Influence On Some Varieties Of Blackberry Cultivars

During The Micro-Cloning Process, Conservation Of Plant Diversity, International Symposium 3rd Edition,2014, 22—24 May 2014, Chijinäu, pp 20.

5. Lozinschii, M, Ciorchinä, N., 2013, The micro-cloning particularities of Thorless Blackberry Cester and Loch

Ness, Journal Of Botany, Vol. V, Nr.l (6): 15-24.

6. Ruzic, D., Lazic, T. , 2006, Micropropagation as Means of Rapid Multiplication ofNewly Developed Blackberry

and Black Currant Cultivars. Agriculturae Conspectus Scientificus, 71(4): 149—153.

MICROPROPAGAREA IN VITRO A UNOR CULTIVARE DE MUR - PERSPECTIVE PENTRU REPUBLICA MOLDOVA

(Rezumat)

In lucrarea datá se descriu conditiile de dezvoltare a unor cultivare de mur farä spini Thornless evergreen, Cester, Arapaho, Polar, Remontana, Thornfree, Smothstem, Loch Ness a speciilor Rubus lacinatus, Rubus canadensis, Rubus fruticosus, in cultura in vitro. A fost stabilit protocolul de sterilizare a materialului initial. Pentru initiere si dezvoltare in vitro a fost selectat mediul de bazä Murashige & Scoog 1962 (MS), cu adaos de 30 g/1 zahär. Mediul optim pentru microclonare este MS 100%, aditionat cu regulator de crestere , 6-benzylaminopurine (BAP) 0.5 mg/1. Rizogeneza mai eficientä s-а obtinut pe mediul MS 50%, cu adaos de 30 g/1 zahär.

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