Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)

Botany

Mariana LOZINSCHII, Nina CIORCHINÄ, Tatiana CALALB

In 35—40 days, on both media, both cultivars developed a white callus formation at the base of the plant, while the root system was less developed. On average, on IBA (0.1 mg/1) medium, Loch Ness reaches 7—8 cm in length and Chester reaches 8—9 cm. When the IBA addition is 0.2mg/l, the shoots reach different lengths, of 8-10 cm for the Loch Ness cultivar and 10-12 cm for the Chester one.

a) b)

Photo 3: a) Smothstem; b) Thornless evergreen Rooted on the medium replenished with NAA

A positive influence on Thornless evergreen cultivar showed the medium filled with auxine. On the medium supplemented with IBA, the roots are devloped as well as a white callus formation, while on the medium supplemented with NAA, the root system develops entirely as well as the plant. To optimize the micropropagation of this cultivar, we used the 50% MS medium with the addition of 30 g sugar, a liquid supplemented with NAA in concentration of 0.1, 0.2 mg 1, on this medium, the sprout grows vigorously up to 12 cm in 30 days and it is rooted in 14 days. The first roots appear in 10—12 days on this medium, during this period the shoot length is 5 cm. In contrast to Chester and Loch Ness, the rootedness here is well-developed (Photo 2). For the Reuben and in particular, for Smoothstem cultivars, an optimum and efficient medium has been established, both for proper growth, and rootedness, with 50% MS composition and 0.1mg/l NAA. On this substrate, the plant axis grew to 8 cm in 30 days, and it was observed that the basal part of the plant had a branched root system, made of 5—6 brached white roots (Photo 3). The plant with it’s increased vigor, has a growth high potential, eventually, its cuttings are further micropropagaed on culture media, while the basal part of the shoot is passed to ex vitro.

Photo 4: Cultivar Polar on culture medium MS 50%

14