Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)
Botany
Micropropagation of blackberry cultivars — perspectives for Republic of Moldova Cultivar Medium % Concentration Growth time Shoots’ number Shoots’ length growth regulator_______________________(mg/1)___________(days)_____________________(cm) Smothstem MS 100 0.3 55-60 14± 1.17 9.1 ± 1.19 0.5 23±0.10 4.9+0.87 ________________________________________07___________________________26±0.09_______3.9+1.10 Reuben MS 100 0.3 55-60 12+0.2 4.7+1.41 0.5 20+0.09 4.2+0.91 ________________________________________07___________________________24+0.20_______2.7+1.05 Polar MS 100 0.3 55-60 15+0.16 8.0+0,15 0.5 24+0.20 6.5+0,25 ________________________________________07___________________________29+0.17_______4.5+0,37 Arapaho MS 100 0.3 55-60 13+0.19 7.5+0.19 0.5 20+0.19 6.1+0.08 ________________________________________07______________________ 26+0.09________3.5+0.08 Thornfree MS 100 0.3 55-60 12+0.11 8.9+0.19 0.5 18+0.13 6.7+0.08 _____________________________ 07 _ 25+0.09_____4.2+0.15 According to table 1: data analysis, it is observed that as we increase the concentration of cytokinins, the shoot length decreases, and when the BAP concentration decreases, the shoot length increases. At a concentration of 0.7 rng/1 BAP, the maximum reached length is 6.9 cm, and at a concentration of 0.3 mg/1, the shoot reaches 10 cm in length. a) Photo 2: Chester (a) and LochNess (b) multiplication This shows what affectes the developing of adventitious buds (Photo 2), the need to increase the BAP concentration that considerably increases the number of shoots. Also, it was being researched the MS medium supplemented with auxine. Chester, Loch Ness and Thornless Evergreen cultivars were used to analyze the MS medium with addition of (IBA). In the research we used 2 different nutrient media with IBA additions, in concentrations of 0.1 mg/1 and 0.2 mg/1. Minimal concentrations of the growth regulator were used not to facilitate the plant’s inhibition. After a period of 21 days, a thickened formation was observed at the shoot base in both cases, for Chester and Loch Ness cultivars. The Loch Ness shoot reached 6 cm in length, while the Chester shoot reached 7 cm in length, on the nutrient media with IBA concentration of 0.2 mg/1, but on the IBA concentration of 0.1 mg/1, the Loch Ness shoot reached 5 cm in length and Chester reached 6 cm. 13 b)
