Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

Glossary

nucleic acid segments which are identical to each other in the various types. These are consensus sequences.

Coverage: This shows how many synthesized DNA strands detect a certain DNA sequence in the course of next generation sequencing.

Degenerate primer: Short nucleic acid segment used at polymerase chain reactions, not all its bases are complementary with the sequence they bind to.

Denaturation: The macromolecules lose their native configuration due to heat, chemical or other effects; in the case of a DNA this means the two strands will untwist.

DISH: (Diffuse Idiopathic Skeletal Hyperostosis) Forestiers disease, the ossification of the ligaments in front of the vertebra.

DNA cloning: The technique with which a specific DNA sequence is put into elements capable of self-replication (plasmid, virus) in order to multiply itself, so that a large volume of copies can be obtained.

Dot/slot blot: Bringing the DNA or RNA isolated from cells to a nitrocellulose or nylon filter, which is made visible with a marked probe. The result is called “dot” or “slot blot”.

Downstream: The position which follows the gene’s 3’-end (its opposite is upstream).

Epitope: Antigene determinant (a few for each molecule).

Formamide: A small, organic molecule that denatures the DNA; it connects to the adenines free

NH2

groups and prevents the formation of A-T basepairs.

Gene: The DNA sequence responsible for coding an RNA and regulating the transcription process.

Genome: The entire set of genetic information of an organism.

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