Hidrológiai Közlöny, 2015 (95. évfolyam)
2015 / 5-6. különszám - LVI. Hidrobiológus Napok előadásai
108 HIDROLÓGIAI KÖZLÖNY 2015. 95. ÉVF. 5-6. SZ. hatást is kifejteni képes baktériumfaj képviselőjének ad otthont. Köszönetnyilvánítás Jelen kutatást az OTKA K83893 számú pályázat támogatásával végeztük. Irodalom Altschul, S. F„ Madden, T. L, Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., Lipman, D. J. (1997) Gapped BLAST and PSI-BLA- ST: a new generation of protein database search programs. Nucleic Acids Research. 1;25(17) 3389-3402. Bansemir, A., Just, N., Michalik, M„ Lindequist, U, Lalk, M. (2004) Extracts and sesquiterpene derivatives from the Red Alga Lauren- cia chondrioides with antibacterial activity against fish and human pathogenic bacteria. Chemical Biodiversity. 1,463-467. Boros, G., Mozsár, A., Petes, K, Mátyás, K, Józsa, V., Tátrai, I. (2012) A busa élőhelye, táplálkozása, növekedése és szaporodása a Balatonban. Hidrológiai Közlöny. 92, 12-14. Finegold, S. M., Vaisanen, M. /... Molitoris, D. R., Tomzynski, T. ./.. Song, Y., Liu, C„ Collins, M. D., Lawson, P. A. 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(1997) Balatoni angolnák kopoltyúrégióinak összehasonlító bakteriológiai vizsgálata. A Balaton Kutatásának 1997-es eredményei, Kiadja a Magyar Tudományos Akadémia Veszprémi Területi Bizottsága és a Miniszterelnöki Hivatal Balatoni Titkársága, pp. 149-152. Martinez-Murcia, A. J., Benlloch, S., Collins, M. D. (1992) Phylogenetic interrelationships of members of the genera Aeromonas and Ple- siomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. International Journal of Systematic Bacteriology. 42,412-421. Miranda, C. D„ Zemelman, R. (2002) Antimicrobial multiresistance in bacteria isolated from freshwater Chilean salmon farms. Science of the Total Environment. 293, 207-218. Muyzer, G., Smalla, K. (1998) Application of denaturing gradient gel e- lectrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in Microbial Ecology. Antonie van Leeuwenhoek. 73, 127-141. 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(1996)Antibacterial abilities of intestinal bacteria in freshwater cultured fish. Aquaculture. 145, 195-203. Sugita, //. Tanaka, K, Yoshinami, M., Deguchi, Y. (1995): Distribution of Aeromonas species in the intestinal tracts of river fish. Applied and Environmental Microbiology. 61, 4128-4130. Tátrai, I., Józsa, V., György, A., Havasi, M., Szabó, 1. (2005) A busa biológiai szerepének és hatásának vizsgálata a Balatonban. A Balaton kutatásának 2004.évi eredményei MTA Budapest. ISSN 1419-1075, pp. 93-101 Tsuchiya, C., Sakata, T, Sugita, H. (2008): Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish. Letters Applied. Microbiology. 46, 43-48. Weisburg, W. G„ Barns S. M„ Pelletier, D. A., Lane, D. J. (1991) 16S ribosomal DNA amplification forphylogenetic study. Journal of. Bacteriology. 173, 697-701. Wu, S„ Gao, T, Zheng, Y, Wang, W, Cheng, Y, Wang, G. (2010) Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco). Aquaculture. 303, 1-7. The first results on the bacterial community structures of the intestinal tracts of the Asian carps living in Lake Balaton Kristóf László, Katalin Jáger, Gergely Krett, Gergely Boros, András Specziár, Andrea K. Borsodi Abstract: Since the last official stocking of filter feeding Asian carps nearly 30 years ago, silver (Hypophtalmichthys molitrix) and bighead (Hy- pophtalmichthys nobilis) carps and their hybrids still occur in large numbers in Lake Balaton. They are high meat yield animals and important components of fish populations by consumption of zooplankton and organic detritus. However, little information is available about their nutritional habits. In the present study bacteriological examination of fore-, mid-and hindgut-section of the intestinal contents of three carps caught in Lake Balaton in September 2011 was performed by using cultivation based and molecular biological methods. An average of 107-l 08 / g CFU values was determined from the intestinal contents independently of the composition of the medium used. Among the strains isolated from the silver carps and identified according to their 16S rDNA sequence analysis, members of species Aeromonas veronii, Brevundimonas vesicularis, Micrococcus endophyticus and Bacillus halmapalus were the most abundant. From the midgut section representatives of Aeromonas veronii, Micrococcus yunnanensis and Roseomonas mucosa, while in the hindgut section Acinetobacter Iwoffii, Paracoccus yeei and Lactococcus garvieae were identified frequently. Analysis of band patterns of 16S rRNA gene sequences, obtained from the examination of the carp intestinal contents by denaturing gradient gel electrophoresis, indicated that bacterial community structures of the fore- and midguts from the same fish showed higher similarities than those of hindguts. Hindgut bacterial communities derived from different individuals formed a distinct similarity group. Keywords:Asian carp, microbial community of the intestinal tract, diversity, cultivation, DGGE.
