Dr. Murai Éva - Gubányi András szerk.: Parasitologia Hungarica 29-30. (Budapest, 1997)

In both formulations, the carrier substance was Culigel BD biodegradable polymer that is characterised chemically as cross-linked polyacrylic acids, partially neutralised as sodium salts and blended with special polysaccharides. Laboratory tests of BTI B 5 GR and BIOPREN BM 5 GR preparations on mosquito larvae As test insects, 3rd and 4th instar (L3 and L4) larvae of Aedes aegypti were used. Fifty larvae (25 in each of two replicates) were placed into water samples taken from untreated control and treated media. The larvae were not fed during the experiment. The residual bioactivity of BTI B 5 GR was studied at concentrations of 5 and 10 ppm (a. i.), while the BIOPREN BM 5 GR formulation was tested at 1, 10 and 20 ppm. The appropriate amounts of granules were added to five litres of tap water in large Erlenmeyer flasks. The granules sank immediately to the bottom of the flasks. These treated media were kept at room temperature (22 °C, no special illumination) for different periods of "ageing". From surface layers, aliquot samples of 2 -100 ml volume were taken initially and at weekly intervals. These samples were then tested in 250 ml glass vessels by introducing the Aedes larvae. To assess the bioactivity of active ingredients released from the granules, the emergence rates of adult mosquitoes were determined at each sample collection. These residual activity tests were conducted until the 12th or 16th week, respectively. Test of the BIOPREN BM 5 GR preparation on mosquito larvae under simulated natural conditions The experiment was carried out with larvae of Aedes cantans. Both young (Li and L2) and old (L3 and L4) larvae were used. Twenty specimens were placed into each of the 3 replicates of the control or treated variants. Plastic basins ("tubs") of 40 x 40 x 20 cm in size were filled with 5-litre amounts of water brought from the natural breeding site of this mosquito species. In addition, the bottom of each basin was covered with leaves taken from the same larval habitat. The temperature of water fluctuated between 17 and 18 °C and its pH value between 7.5 and 8.0. The BIOPREN BM 5 GR formulation was tested at a concentration of 0.5 ppm (a. i.). Altosid pellets serving as a reference preparation were tested at 1 ppm (a. i.). Two parallel series of experiments were conducted, one with Li and L2 stage larvae, the other with L3 and L4 instar larvae. The numbers of surviving and dead larvae and pupae as well as those of emerged adults were recorded at 48-hour intervals for 5 weeks. Test of the BIOPREN BM 5 GR preparation on mosquito larvae in closed containers In these studies 3rd instar (L3) larvae of Aedes aegypti were used as test insects. Fifty larvae per replicate were used (3 replicates in the treatments and 2 replicates in the control). Fifty-litre quantities of a mixture of tap water, deionised water and sewage water at a ratio of 3:1:1 were filled into large containers of 75-litre volume. The containers were kept tightly closed with a lid in order to prevent evaporation of water. They were placed into boxes of insulation where the temperature was regulated to maintain a constant level of 28 °C. After a period of 24 hours for the stabilisation of temperature, Aedes larvae were