Dr. Murai Éva - Gubányi András szerk.: Parasitologia Hungarica 29-30. (Budapest, 1997)

added to the containers. Soon thereafter treatments were carried out with the BIOPREN BM 5 GR formulation by throwing the granules onto the water surface where they immediately sank to the bottom. 0.1 and 0.5 g of granules per container were tested corresponding to 2 ppm and 10 ppm (a. i.) concentrations. Further groups of 50 larvae were placed into the containers at weekly intervals. For the evaluation and observation of adult emergence, living pupae were removed daily with a sterile pipette and transferred into cups with water from their containers. Dead, floating larvae were also removed daily. In order to determine the residual efficacy of granules, the survival until normal flying adults was assessed regarding each consecutive evaluation time. Laboratory test of the BIOPREN BM 5 GR preparation on house fly larvae Freshly collected pig manure was used as the rearing medium for fly larvae. In order to eliminate natural insect populations, this medium was first kept at -18 °C for 48 hours. After warming up to room temperature, 4 kg quantities of samples were spread out on aluminium trays to obtain an approx. 3 cm thick layer on each tray. These trays were kept at 15 °C. BIOPREN BM 5 GR formulation containing 5% methoprene was sprinkled uniformly onto the surface of the rearing medium to give final concentrations of 10, 20 and 50 mg a. i./ kg manure, respectively. In one of the experimental series the treated medium was kept in its original state ("dry" treatment), while in the other series the surface was thoroughly wetted after the application of granules ("wet" treatment). At the start of each trial and at 2-week intervals until the end of experiment (10th week), in both treatment variants and at each concentration, 5 x 50 g amounts of manure were measured into plastic containers. Twenty first instar larvae of Musca domestica were added to each of these samples. The containers were sealed with gauze sheets and kept at 25 °C. Untreated media with larvae served as control. After 6 to 8 days any newly formed pupae were removed and placed into glass bottles containing a layer of wet sawdust at the bottom. At the end of each experiment the numbers of pupae and adult flies which had emerged from the pupae were recorded. Test of the BIOPREN BM 5 GR preparation on house fly larvae in pig-rearing houses The experiment was carried out in three closed rooms of the pig-rearing houses of Komárom Agricultural Co. Ltd. in Bartusekpuszta. In these rearing houses, a battery cage management system was used. Each room had 5 rows of 7 battery cages and each cage was equipped with a self-feeder and two automatic water supplies. On average, 8 weaned piglets were placed into a cage one week before the start of each experiment. The manure and urine fell into a collection pit (1.7 x 9.1 m per row). Before the experiment, no fly control with conventional adulticides was performed in the rooms. The study was conducted for 7 weeks and designed so that the 3 rooms were treated in different ways. In Room 1 potential fly breeding sites were treated with BIOPREN BM 5 GR twice at a 3-week interval (in weeks 2 and 5). Room 2 was treated the same way as Room 1 but, in addition, an aqueous solution of PROTECT-B (containing 1% methomyl insecticide and 0.2% tricosen sex attractant) was applied to some surface parts of the cages. Room 3 was untreated and served as control.