Dr. Murai Éva szerk.: Parasitologia Hungarica 24. (Budapest, 1991)

After the publication of Näsmark's (1937) monograph on amphistomes, the structure of the muscular organs (pharynx, genital opening, acetabulum) became one of the most important specific characters in modern amphistome diagnosis. The use of these improved characters allows a correct identification of species, which is the aim of this paper. MATERIALS AND METHODS The test material was collected from the following host species: Salamandra salamandra, Triturus cristatus, T. vulgaris, Bombina bombina, B. variegata, Pelobates fuscus, Hyla arborea, Rana esculenta (s.str), R. ridibunda, R. arvalis, Bufo bufo and B. viridis. Of the wild ruminants Cervus elaphus, Capreolus capreolus, Dama dama and Ovis musimon were examined. Of the domesticated animals, amphistomes were collected from Bos primigenius f. taurus, Ovis ammon f. aries, Bubalus amee f. bubalis. Details on the infected species and their distribution will be described under the individual species. After removing them from the infected organs the samples were treated and whole mounts as well as histological sections were prepared as described elsewhere (Sey 1991). RESULTS AND DISCUSSION AMPHISTOMES RECOVERED FROM AMPHIBIANS Diplodiscidae Skrjabin, 1949 Diplodiscus subclavatus (Goeze, 1782) Diagnosis: Body length 2.1-3.5 mm, body width 1.1-1.7 mm. Acetabulum 0.811.36 mm in diameter. Diplodiscus type, number of muscle units, d.e.c. 23-25; d.i.c, 52-55; v.e.c. 17-19; v.i.c. 54-57; m.e.c, 8-10. Pharynx 0.34-0.43 mm long, Subclavatus type; esophagus 0.14-0.25 mm in length. Testis 0.37-0.39 by 0.28-0.42 mm. Cirrus sac 0.18-0.20 by 0.01-0.01 mm. Ovary 0.18-0.21 by 0.13-0.15 mm. Terminal genitalium Scloeroporum type. Genital pore bifurcal. Eggs 0.123-0.138 by 0.082-0.097 mm. Biologv: Not completely studied. When examining intermediate hosts of amphistomes Sey (unpublished data) often found cercariae of this species in Planorbis píanorbis. Habitat: rectum Hosts: Rana esculenta, R. ridibunda, R. temporaria, R. arvalis, Bufo viridis, B. bufo, Natrix natrix. Natrix natrix is probably the paratenic host. Distribution. In all places where such examinations were caried out; including Szeged, Eger, Hajdúszoboszló, Kapuvár, Hanság, Bikal, Kiskunság National Park, Balaton. References. Margó 1879; Edelényi 1942,1960; Sey 1964; Murai et al. 1986.