Dr. Murai Éva szerk.: Parasitologia Hungarica 11. (Budapest, 1978)
PAULUZZI, DOTTORINI, FRONGILLO (1972a) Found that fractionating hydatid fluid on a Bio-gel P 300 column did not produce fractions that protected Balb/C mice against experimental peritoneal infections. PAULUZZI, DOTTORINI (1972) By further analysis of the fractions from a Bio-gel P 300 column, showed two groups of substances, one of a high molecular weight, which was active in IF and IHA tests, and a second of low molecular weight. PAULUZZI, DOTTORINI, PIRAS (1972b) Using ultracentrifugation of the globulin fraction of hydatid fluid in a sucrose gradient, found that one of two fractions obtained inhibited the activity of the IHAT test. POZZUOLI, MUSIANI, ARRU, PLANTELLI, MAZZARELLA (1972) With Sephadex G-200, isolated a 400 000 and 150 000 molecular weight component from sheep hydatid fluid. BENEX (1972) By fractionating an extract of Taenia saginata on a Sephadex G-200 column, found that three fractions cross reacted with sera of patients with hydatid disease in the DDG, CFT, and LAT. VARELA-DIAZ, COLTORTI, RICARDES, GUISANTES, YARZABAL (1974) Found antigen for Band 5 in 40 of 42 hydatid cyst pools. RUSSI, SIRACUSANO, VICARI (1974) Found that a glycoprotein isolated from hydatid cyst membrane with a high P^ blood group activity was active in 11 of 21 sera from patients with pulmonary cysts. POZZUOLI, MUSIANI, ARRU, PATRONO, PIANTELLI (1974) With specific immunosorbents, absorbed sheep hydatid fluid for removal of host components on a Sepharose 4B column. Two major antigens, one of molecular weight greater than 400 000, was more immunoreactive than a smaller molecular weight component of 150 000. FEIZI, RABAT (1974) Isolated P^ blood group substance plus I material from sheep hydatid ' fluid by immunoabsorption. AL CONTRERAS, KNIERIM (1974b) Determined protein and carbohydrate antigens in five lots of hydatid fluid. Protein varied from 14 to 65% and carbohydrate from 1.4 to 4. 5%. Although two hydatid pools had similar protein and carbohydrate contests, only one was sensitive in the IEP test. BOUT, FRUIT, CAPRON (1974) With two dimensional Immunoelectrophoresis , isolated the antigen responsible for the specific Band 5 in the IEP test. Monospecific antiserum to Band 5 was made. Use of this antiserum should facilitate the specific diagnosis of hydatid infections. The Band 5 antigen is a lipoprotein with a molecular weight of 60,000 and is similar to antigen A reported by Oriol et al. (1971). POZZUOLI, PIANTELLI, PERRUCCI, ARRU, MUSIANI (1975) With affinity chromatography on concanavalin A-Sepharose, isolated two specific antigens (Antigen 4 and 5). Antigen 4 was found in 58 of 75 (77%) hydatid sera by the IEP test, in 81% by CEP tests, and in 89% by IHAT. ROMBERT, FRAGO de AZEVEDO (1975) In a comparative study of hydatid fluid scolex and membrane antigens in serologic tests, found that membrane antigens were inactive, purified scolex antigens were active at the LAT and IHAT with low titers, and scolex antigen was active in the DDG test. Since scolex antigens detected antibodies that were not detected in the LHAT, the authors recommend the use of both antigens. YARZABAL, DUPAS, BOUT, CAPRON (1976) By using a monospecific anti-Band 5 antiserum, localized the antigen 5 by the IFT in the inner portion of the germinal membrane and in the parenchyma of the protoscoleces.
