Dr. Murai Éva szerk.: Parasitologia Hungarica 11. (Budapest, 1978)
Table 6 Countercurrent Electrophoresis (CEP) SORICE, CASTAGNARI (1971) Reported the CEP to be slightly more sensitive (79%) than the IEP (63% CASTAGNARI, SORICE (1971b) Reported the CEP to be more sensitive than the IHAT, LAT, and EEP test. LECUBARRI, CORROLLER, BE LKAK) (1971) Compared the CEP test with the LAT ina study of 160 patients with hydatid cysts in Algeria. The test was 97% specific and 80% sensitive. CASTAGNARI, SORICE (1971b) Reported that the CEP test gave 94. 7% sensitivity. With IEP, 8 specific bands were recognized, and 22 of 28 cases (78. 5%) were diagnosed. In CEP, 4 specific bands were recognized, and 18 of 19 cases (94.7%) were diagnosed. In a comparison with the IEP test, the IHAT, and the LAT, the CEP test was most sensitive. The authors recommend IHAT and CEP for diagnosis. GENTILINI, PINON (1972) Using cellulose acetate membranes in the CEP test, compared the technique to DDG and IEP. Of 40 confirmed hydatid cases, 38 were positive by HSP and CEP. More bands were visualized in CEP then IEP. "Band 5" was present in all positive sera. TORRES, GUISANTES, ALVAREZ, YARZABAL (1973) Reported good sensitivity and specificity in the CEP test, with concentrated serum. GEORGIE VA- BO J AD ZIE VA , DASKALOVA, STAROVA, BOJADZIEV, DEJANOV (1975) Compared CEP with CFT and found CEP to be more sensitive. LOPEZ-LEMES, VARELA DIAZ (1975a) Found CEP less specific than IEP, since "Band 5" could not be readily detected, SORICE, DELIA, CASTAGNARI (1975a) Found CEP, with concentrated hydatid fluid antigen, more sensitive than the IHAT. APT (1975) Reviewed diagnostic methods. KELKAR, KOTWAL (1975) Found 4 of 6 sera positive in CEP tests, 1 of 6 in DDG tests, and 2 of 5 in the IDT. DRAPER (1976) Reviewed diagnosis of parasitic infections by CEP. PINON (1976) Compared CEP to IEP and IHAT. CEP tests were positive in 81.4% of 400 cases of hydatid disease, and IEP tests in 76. 6%. Seventy-five percent were positive in both. IHAT were positive in 91.8%. A characteristic "gloved finger" pattern in CEP indicates a cyst ready to burst. Table 7 Antigens ORIOL, WILLIAMS, PEREZ ESANDI, ORIOL (1971) Isolated two lyoprotein antigens (A and B) from sheep hydatid fluid with minimum (1.6%) host serum proteins. One component (B) was obtained pure by gel diffusion. WILLIAMS, PEREZ ESANDI, ORIOL (1971) Found antigen A and B to be reactive in IHAT, the IEP test, and the IDT. The antigens, however, were not as sensitive as crude hyd-l atid fluid. MUNTIAN (1971) Examined the antigenic composition of hydatid fluid (HF), scolices (SC), and germinative membrane (GM). HF contained 10 antigens; SC, 17 antigens; and GM, 8 antigens. FRONGILLO, DOTTORINI, PERFETTI (1971) Fractionated hydatid fluid with a Bio-gel P 300 column. IHAT activity was found in the first 15 tubes containing protein, but CF activity was found in almost all fractions.
