Dr. Murai Éva szerk.: Parasitologia Hungarica 11. (Budapest, 1978)

QUILICI, ASSADOURIAN, RANQUE (1971) Evaluated the CFT, LAT, IHAT, IFT, and IEP test on 68 proven cases in southern France. The nonspecificity was 8, 7, 6, 4, and 0%, respectively

. Authors recommend either IEP and CF or IHAT and IF tests for diagnosis. CASTAGNARI, SORICE (1971a) Compared specificity of IEP and countercurrent electrophoresis (CEP). Although CEP was more sensitive, the authors stressed the importance of using several tests in diagnoses. CONTRERAS, KNIERIM (1974a) Evaluated IEP, DDG, and the IHAT in 67 patients with hydatid disease. The sensitivity of the tests were 56.7%, 48.2%, and 71.6%, respectively. Because of its clinical difficulties, the IEP test was not recommended for diagnosis. VARELA-DIAZ, COLTORTI (1974a) Described techniques for performing the IEP, IHAT and LAT. YARZABAL. LEITON, LOPEZ-LEMES (1974) Found the IEP more sensitive than the IHAT in the preoperative diagnosis of pulmonary hydatidosis in 54 patients. COLTORTI, VARELA-DIAZ U975) Modified the IEP by substituting normal serum for concentrated serum and filling the serum throughs several times. GUISANTES, YARZABAL, VARELA-DIAZ, RICARDES, COLTORTI (1975) Proposed a standardized test for IEP, with 0.9% agarose over 1% agar used as a support medium. Rectangular wells were superior to circular wells. The optimal concentrate of crude hydatid fluid was 200 mg dry weight/ml and for purified hydatid cyst fluid antigen, 30 mg protein/ml. LOPEZ-LEMES, VARELA-DIAZ (1975a) Detected Band 5 in 45% of 51 sera. Treating the slice with citrate brought out Band 5 more clearly, as did staining with Amino-Schwartz. VARELA-DIAZ, GUISANTES, RICARDES, YARZABAL, COLTORTI (1975c) Found that although purified hydatid cyst antigen was more sensitive than crude cyst fluid antigen, the former was more nonspecific. Bands other than "Band 5" may be useful in the diagnosis of hydatidosis. YARZABAL, RETAMAL, SEPULVEDA, GUACHALLA, KIGUEL (1975a) In 71 surgically confirmed patients with pulmonary hydatidosis in Chile, found the IEP positive in 55 (77.4%). The IEP is recommended for diagnosis, with IHAT and IFT as comparison tests. YARZABAL, SCHANTZ, LOPEZ-LEMES (1975b) Compared the IDT with the IEP in 47 surgically confirmed cases. The IDT gave a few false positive reactions, but the IEP gave none. The high specificity of the IEP led these workers to recommend this technique over the skin test. RAZZOKOV (1975) Evaluated DDG and IEP for species-specific components. Eight speciesspecific bands were found in human sera from patients with E. granulosus, of which 2 were diagnostically important. Species-specific bands for E. multilocularis were also found. SCHANTZ (1976) Recommended that the IEP be included among those tests used for diagnosis of hydatid disease because of the presence of the specific "Band 5". VARELA-DIAZ, COLTORTI, PREZIOSO, LOPEZ-LEMES (1975b) Evaluated the IEP test, LAT, and IHAT. The sensitivities of the LAT and IEP test were comparable; the IEP test gave a higher sensitivity than the IHAT. The LAT and IHAT, with tannic acid-treated cells, are recommended for screening, with the IEP as the test for use in confirming positive reactors. RICKARD, ARUNDEL (1976) Reported that the program of Varela-Diaz et al. (1975) for the diagnosis of hydatid disease is being offered in Australia by the University of Melbourne, Veterinary Clinical Center.