Dr. Murai Éva szerk.: Parasitologia Hungarica 11. (Budapest, 1978)

REISSENWEBER, LOZANO, SAMPIO, MUNIZ, ACEVEDO (1973) Using blood collected on filter paper and venipuncture, compared 76 paired samples in the LAT, IFT, and CEP test. The results obtained with the filter paper eluates were usable in all tests. GENIS (1974) Studied the prevalence of hydatid disease in Kazakhstan; tested 1 689 persons by the LAT. The technique was found to be a useful method for seroepidemiologic study. GUISANTES, VARELA-DIAZ (1975) Compared the LAT to the DDG and the IEP tests. These authors recommend the LAT as a diagnostic screening test because of its high specificity. ZORIKHINA, BREGADZE (1975) Found that titer by LAT began to fall a few months after operation for removal of hydatid cyst and that it became negative in 1 to 3 years. HOGHOOGHI, SABBAGHLAN, GH AD IRIAN, KAGAN, SCHILLER (197 6) Employing IDT and LAT, 51 patients were evaluated. The LAT was 82% sensitive and 5% nonspecific. Table 4 Immunofluorescence Tests (IFT) GORE, SADUN, HOFF (1970) Employed acetate impregnated with E. granulosus antigen fractions and developed a test that could be read by a fluorometer; it had a sensitivity of 82% and a nonspecificity of 4%. AMBROISE-THOMAS, KEEN TRUONG (1970a, b) Evaluated 300 proven cases and reported a sensitivity of 96% for the IFT. Sera of patients with extirpated cysts became negative in 12 months. RUITENBERG, van der SLEEN (1972) Reported a method of attaching whole scolices to glass slides. KANE, MATOSSIAN, BATTY (1971) Utilizing monospecific IgG, IgM, and IgA in 9 cases of hydatid disease, found IFT positive for IgG in 8, for IgM in 4, and IgA in 6. The IHAT was positive in 8 of 9 cases, the CFT in 5 of 9 cases. HULDT, LJUNGSTROM, AUST-KETTIS (1975) Found class-specific conjugates for hydatid infection to be useful in the diagnosis of infections in a population of Lapps in Scandinavia. In a group of 50 infected Laplanders, 60% showed IgM reactivity. AMBROISE-THOMAS (1976) Reviewed immunofluorescence for the diagnosis of parasitic diseases. FLOREZ, SANCHEZ, ALBALA (1976) Found IFT to be 90% sensitive in diagnosis. WIKERHAUSER, DZAKULA, KUTICIC (1977) Using cryostat sections of protoscolices of E. granulosus, found 16 of 18 proven cases positive in the IFT. Table 5 Immunoelectrophoresis (IEP) CAPRON, YARZABAL. VERNED, FRUIT (1970a) Evaluated 400 sera by CFT, IHAT, LAT, IFT, and IEP test and found the IEP test to be the most sensitive (85%). Sera from patients with hydatid cysts due to E. granulosus had a 90% reactivity, and those with lung cysts, a 69% reactivity. CAPRON, VERNES, FRUIT (1970b) In tests with 7 patients with E. multiocularis disease, identified a specific band for this parasite. YARZABAL, CAPRON (1971) Reported very high specificity for Band 5 in human sera. YARZABAL (1973) Compared DDG, IEP, and CEP and found IEP to be the method of choice for diagnosis.