Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)

Botany

L. ROMAN, A. HOSU, C. VASILIU, H. ROMAN, Gh. CIUCÄ, G. MIHÄESCU

distribution process in several stages. This process includes: an adsorbent (stationary phase), a solvent or mixture of solvents (mobile phase or eluent) and the sample molecules.

As support for the thin layer, we used an aluminum absorbent foil (20 x 10 cm) covered with silica gel 60F254 HPTLC into strips 8 mm to 1,5 cm from the lower edge of the plate at a rate of 80 nL / s, using a semi-automatic applicator device (Linóm 5 - CAMagic, Muttenz, Switzerland).

The fingerprints detected at different wavelengths obtained after development, have allowed the observation of various features for complete information.

Results and discussion

The spectrum of virulence strains studied

The resistance factors for the spectral analysis of the 10 strains analyzed (4 strains of Bacillus subtilis 3 strains of Bacillus pumilus and 3 Pseudomonas syringae strains) revealed the presence of external enzymes and proteases pore-forming. Gelatinase activity of matrix metalloprotease present, giving strains producing capacity of invasion and dissemination in the wood structure.

a

b c

d

Fig. 1: Detection of the external enzymes: gelatinase (a), caseinase (b) DN-ase (c) and lecitinase (d)

MICs read at an absorbance of 620 nm was calculated as the lowest concentration which inhibits by 50% the increase of CFU (compared to the positive control of well 11). In the positive control was made sowing of bacterial suspension without added plant extract.

Table 2: Absorbance value of UFC corresponding hydroethanolic concentration of Juglans nigra extract observed at spectrophotometer reading at 620 nm.

____________UFC (observed at 620 nm)_______________________________________________________

Concentr Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus Pseudomonas Pseudomo- Pseudomonas of 1. nigra subtilis 2 subtilis subtilis subtilis pumilus pumilus syringae 1\ nas syringae syringae 21

______________III. 4L 25 11. 29 II. 311. 161. III.________8JL_________III.

500 mg/ml 0.121 0.098 0.078 0.179 0.107 0.096 0.087 0.064 0.094

250 mg/rnl 0.098 0.064 0.069 0.095 0.086 0.056 0.067 0.057_______0.059

125 mg/ml 0.056 0.081 0.076 0.049 0.076 0.051 0.121 0.051_______0.063

62.5 mg/ml 0.080 0.084 0.095 0.058 0.083 0.068 0.149 0.089 0.096

31.25 mg/ml 0.098 0.107 0.099 0.078 0.095 0.097 0.198 0.129 0.131

15.625 mg/ 0.168 0.178 0.207 0.097 0.167 0.174 0.209 0.183 0.159

ml_____________________________________________________________________________________________

7.831 mg/mf 0.121 0.108 0.078 0.259 0.271 0.169 0.328 0.478 0.149

3.90 mg/ml 0.253 0.476 0.498 0.287 0.296 0.375 0.427 0.309 0.375 ~

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