Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)

Botany

Antibacterial activity ofcompouds derivedfrom hidroethanolic extracts of Juglans nigra

1.953 mg/ml 0.392 0,497 0.652 0.318 0.379 0.399 0.357 0.391 0.401

0.976 mg/ml 0,409 0.597 0.714 0.375 0.487 0.439 0.524_______0.549______0,523

M* (without 0.599 0.579 0.609 0.578 0.586 0.594 0.593 0.592 0.592

extract)_____________________

M“(without 0.048 0.046 0.046 0.048 0.047 0.045 0.048 0.045 0.048

bacterial

inoculum)

MCI of the hydroethanolic extract of J. nigra had values between 15,625 pg / mL and 250 pg /mL.

В BacillussuixSis 2HI.

■ SattfAjssLiDttfii: 25 ii в BacillusритйивЗ It.

Я Pseudomonas, syringae 11 ili. В Pseudomonas syringes 21 Hi.

Я BacillussuixSis 4L Bacillus subtilis 29IL в Bacillus pumSus 16 L В Pseudomonas syringae Síi.

Fig. 2: Effect of Juglans nigra hydroethanolic extract against phytopathogenic bacteria.

Separation of the secondary compounds of J. nigra extract with antibacterial activity by thin-layer chromatography (TLC)

Chromatographic fingerprints read at different wavelengths revealed the existence in the composition of the J. nigra extracts of compounds with antibacterial activity. The best resolution was obtained at an absorbance of 400 nm.

A

B

C

Fig. 2 Chromatographic Fingerprints of Juglans nigra hydroethanolic extract: (A) detection at 254 nm, (B) visible light detection and (C) detection at 400 nm. The sequencing of standard plates and extract compounds: 1 - ferulic acid, gallic acid 2, 3 - chlorogenic acid, quercetin 4-, 5- plant extract, rutin 6, 7 - kaempferol

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