Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

CHAPTER EIGHT – PCR and NGS investigations

Figure 38. D7S818 marker,

reverse

sequence,

CTAT

motifs are in brackets. In the area marked by the arrow, on the -3’ end of the reverse strand, an A>G sequence variation can be found very close to the repeating sequences, which significantly inhibited hybridization of the reverse primer and caused the result to be of limited value.

D19S433: Analysis of this marker was performed by János

Molnár, biologist-bioinformaticist, who ended up facing many difficulties. He found that duplicated or shorter/longer flawed reads and sequence variations occurred quite often. For this reason, he handled the reads individually, while ignoring base differences (sequencing errors) and only taking fully matching ones into account. The M1 and M2 samples of skeleton 11/52 were much easier to analyze. 12/13 genotypes were identified. The Ml and M2 sequences taken into account are the following:

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