Hidrológiai Közlöny, 2015 (95. évfolyam)

2015 / 5-6. különszám - LVI. Hidrobiológus Napok előadásai

72

HIDROLÓGIAI KÖZLÖNY 2015. 95. ÉVF. 5-6. SZ.

No

Vízfolyás

megnevezése

Mintahely

GPS koordináta

DRO

LNG

PRG

1.

Zala folyó

Őriszentpéter

N46.83666, E16.43392

1.85 (0.04)

3.40(0.45)

3.25 (0.59)

2.

Zala folyó

Zalalövő

N46.85013, E16.62666

<LOQ

<LOQ

2.74 (0.12)

4.

Zala folyó

Petőhenye

N46.88008, El6.92030

<LOQ

<LOQ

2.92 (0.33)

5.

Zala folyó

Zalabér

N46.97823, El7.02623

<LOQ

<LOQ

2.81 (0.25)

7.

Zala folyó

Balatonhídvég

N46.63705, E17.18369

1.54(0.03)

1.29 (0.09)

10.17(1.17)

9.

Denke patak

Pankasz

N46.83737, E16.49827

<LOQ

<LOQ

1.47 (0.16)

10.

Szentjakabi csatorna

Felsőjánosfa

N46.84740, E16.55136

<LOQ

<LOQ

0.52 (0.04)

12.

Szélvíz

Zalaszentgyörgy

N46.86587, E16.69467

<LOQ

<LOQ

0.73 (0.14)

14.

Felső-Válicka

Zalaegerszeg

N46.84334, E16.86976

<LOQ

<LOQ

4.91 (0.24)

15.

Sárvíz

Zalaszentiván

N46.89049, E16.89153

<LOQ

<LOQ

4.41 (1.31)

21.

Héviz-páhoki csatorna

Alsópáhok

N46.76038, E17.20494

1.62 (0.05)

1.26 (0.01)

2.28 (0.34)

20.

Sári csatorna

Marcali

N46.58546, El7.44281

<LOQ

2.14(0.04)

1.38 (0.03)

22.

Edericsi patak

Balatonederics

N46.80127, E17.38797

<LOQ

0.85 (0.07)

<LOQ

27.

Egervíz

Szigliget

N46.80661, E17.45916

<LOQ

<LOQ

1.81 (0.03)

28.

Egervíz

Hegyesd

N46.91531, E17.52500

<LOQ

<LOQ

1.88(0.07)

29.

Egervíz

Monostorapáti

N46.92733, El7.55878

<LOQ

<LOQ

2.37(0.03)

31.

Örvényesi-séd

Örvényes

N46.91394, E17.81708

<LOQ

<LOQ

0.23 (0.02)

32.

Aszófoi-séd

Balatonfüred

N46.93233, E17.85875

<LOQ

<LOQ

1.00 (0.01)

37.

Kéki patak

Balatonalmádi

N47.04098, El8.02046

<LOQ

<LOQ

2.27 (0.10)

42.

Boronkai patak

Marcali-Boronka

N46.58641, E17.44498

0.26(0.01)

<LOQ

2.07 (0.01)

43.

Imremajori csatorna

Balatonfenyves

N46.70956, E17.47938

4.30 (0.30)

2.45 (0.01)

13.67(0.31)

1. táblázat - Progestin pozitív mintahelyek a Balaton és a Zala vízgyűjtőn. A táblázat első négy oszlopa mutatja a mintahely számát (No), a vízfolyás és a mintahely megnevezését a GPS koordinátáikkal együtt. Az utolsó három oszlop az átlagos progestin koncentrációkat mutatja ng/L-ben. Zárójelben a S.E.M. értékek vannak feltüntetve.

Detection of anthroponetic steroid contaminations in catchment area of Zala River and Lake Balaton by HPLCMS/MS method

Z Pirger'“, G Maasz'“'3, P Takács,h, S Lovas'“, Z Zrínyi'“, R Svigruha2, A Takátsy3, L G-Tóth'c, P Avar'

1- MTA Centre for Ecological Research, Balaton Limnological Institute, a- Department of Experimental Zoology, NAP B Adaptive Neuroethology, b- Department of Hydrozoology, Fish and Bentos Research Group and c- Zooplankton and Perifhyton Theme Group, Tihany, H8237, Hungary; 2- Limnoecology Research Group, Department of Limnology, University of Pannónia, Veszprém, H-8200, Hungary; 3- Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of Pécs, Pécs, H-7624, Hungary

Abstract: Freshwaters, such as lakes and rivers, today are subject to controlled pollution. Steroid hormones are chemically very

stable, highly lipophilic molecules and their biological properties have a strong impact on the endocrine regulation of invertebrate and vertebrate species. Steroids have estrogenic, androgenic and progestogenic influence and based on it, they could modify the normal physiological mechanisms of freshwater species by mimicking the effect of endogen steroids. In this study, we focused on synthetic progestins as they are the main active ingredients of contraceptive pharmaceuticals. Progestins have been shown to impair reproduction in fish, amphibians and mollusks at low ng/L concentrations. In our measurements, we focused the most frequent oral contraceptive substances, such as levonorgestrel (LNG), drospirenone (DRO) and progesterone (PRG). We developed and optimized a quick, sensitive and relative cheap liquid chromatographic - mass spectrometric method with solid phase extraction to measure the hormone levels. Using our sensitive method (LOQ 0.03-0.11 ng/L) we could also detect progestins even between 0.1 and 1 ng/L concentrations. Analyzing freshwater samples from Lake Balaton catchment area we found influents where the concentration of these hormones were 0.26-4.30 (DRO), 0.85-3.40 (LNG) and 0.23-13.67 (PRG) in ng/L. Out of 53 collecting places 21 contained measurable progestin levels, which clearly demonstrates the applicability of our method, legitimates toxicology experiments on effected species and indicates monitoring efforts.

Keywords: levonorgestrel, drospirenone, progesterone, catchment area of Lake Balaton and Zala River, HPLC-MS/MS.