Hidrológiai Közlöny 2008 (88. évfolyam)

6. szám - IL. Hidrobiológus Napok: „A Balaton és vízrendszere – a Balaton-kutatás története” és „A Duna-kutatás története” Tihany, 2007. október 3–5.

117 Irodalom AFNOR (1996): Determination of the inhibitory effect on the growth of Lemna minor. - XP T 90-337. Aida, M„ Itoh, K„ Ikeda, H„ Harada. N„ Ishii, Y„ Usui, K. (2004): Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl. - Weed Biology and Management 4: 127-135. ASTM (1991): Conducting static toxicity tests with Lemna gibba. Guide E 1415-91. ASTM International, Philadelphia, U.S. ASTM (2004): Standard Guide for Conducting Static, Axenic, 14-Day Phytotoxicity Tests in Test Tubes with the Submersed Aquatic Macrophyte, Myriophyllum sibiricum Komarov. - Guide E 191304. ASTM International, Philadelphia, U.S. Bennicelli, R., Banach, A., Szajnocha, K., Ostrowski, J. (2004): The ability of Azolla caroliniana to remove heavy metals (Hg(II), Cr(lll), Cr(VT)) from municipal wastewater. - Chemosphere 55: 141-146. Brown, I. (1994): OSS procedure for the biological testing of waters in tropical Australia. Aquatic fern test (Azolla pinnata). - Internal report 163, Supervising Scientist for the Alligator Rivers Region, Canberra. Unpublished paper. Chattopadhyay, A., Adhikari, S., Adhikary. S.P., Ayyappan, S. (2006): Evaluation of butachlor for control of submerged macrophytes aIong with its impact on biotic components of freshwater system. Iran. J. Environ. Health. Sei. Eng. 3: 103-108. Eberius, M. (2001): Observation Parameters in the Duckweed Growth Inhibition Test. - http://www.lemnatec.com/Literatur/LT005.pdf Environment Canada (1998): Biological test method: test for measuring the inhibition of growth using the freshwater macrophyte Lemna minor. - Report EPS l/RM/37. EPA (1996): Ecological effects test Guidelines OPPTS 850.4400 Aquatic plant toxicity test using Lemna ssp., Tiers I and II. - EPA 712C-96-156. Software-based asessment of leaf area in ecotoxicologieal tests Kováts, N- - Császár, G. - Borbély, G. - Paulovits, G. Abstract: The Lemna minor growth inhibition test is widely used in ecotoxicology, the standard is to be introduced in Hungary (ISO/FDIS 20079: Water quality - Determination of the toxic effect of water constituents and waste water on duckweed /Lemna minor/ - Duckweed growth inhibition test). According to available test protocols, growth can be assessed on the basis of frond number, wet weight and total frond surface. The Duckweed Detector software was developed at the Department of Image Processing and Neurocomputing of the University of Pannónia for surface measurements in Lemna tests but can be used for other test organisms as well. In this study Azolla was assessed, an exotic species in Hungary, which can primarily detect pesticide toxicity. The software was calibrated to be used for evaluation of water fern growth inhibition in bioassays. Keywords: Lemna minor, Azolla caroliniana, reproduction inhibition, surface measurement, visual assessment. ISO/FDIS (20079): Water quality - Determination of the toxic effect of water constituents and waste water on duckweed (Lemna minor). Duckweed growth inhibition test. OECD (2002): Guidelines for the Testing of Chemicals: Lemna sp. Growth Inhibition Test, Draft Guideline 221 OECD (2006): Guidance Document on Simulated Freshwater Lentic Field Tests (Outdoor Microcosms and Mesocosms). - OECD Series on Testing and Assessment, No. 53, Paris. Roshon, R.D., McCann, J.H., Thompson, D.G., Stephenson, G.R. (1999): Effects of seven forestry management herbicides on Myriophyllum sibiricum, as compared with other nontarget aquatic organisms. - Can. J. For. Res. 29(7): 1158-1169. Sela, M„ Tel-Or, E., Fritz, E„ Huttermann, A. (1988): Localization and toxic effects of cadmium, copper and uranium in Azolla. Plant Physiol. 88: 30-36. SIS (1995): Swedish Institute of Standards, Water quality-determination of growth inhibition (7-d) Lemna minor duckweed. SS 02 82 13. Smrchek, J., Clements, R., Morcock, R., Rabért, W. (1993): Assessing Ecological Hazard Under TSCA: Methods and Evaluation of Data. In: Environmental Toxicology and Risk Assessment, ASTM STP 1179, Wayne, G.L., Hughes, J.S. and Lewis, M.A. (eds), American Society for Testing and Materials, Philadelphia, pp. 22-39. Vervliet-Scheebaum, M., Knauer, K., Maund. S„ Grade, R.. Wagner, E. (2006): Evaluating the necessity of additional aquatic plant testing by comparing the sensitivities of different species. - Hydrobiologia, 570: 231-236. Zhihui, S., Tianyi, Ch. (1998): Toxicity of Tributyltin to Lemna minor L. and Azolla filiculoides Lamk. - Bull. Environ. Contam. Toxicol. 60: 318-322. Klossy Irén alkotása