Hidrológiai Közlöny 2007 (87. évfolyam)
6. szám - XLVIII. Hidrobiológus Napok: Európai elvárások és a hazai hidrobiológia Tihany, 2006. október 4–6.
185 HIDROLÓGIAI KÖZLÖNY 2007. 87. ÉVF. 6. SZ. egységnyi fehérjére vonatkoztatott ún. specifikus aktivitás felülmúlta a még vizsgált toxintermelő cianobaktériumok kivonatánál tapasztalt proteáz aktivitást. Összefoglalás Munkánkban a hajdúszoboszlói Prymnesium parvum vízvirágzás vízkémiai hátterét vizsgálva arra a következtetésrejutottunk, hogy a víztérben bekövetkezett tömeges halpusztulást minden bizonnyal az elszaporodott haptofiton szervezet mérgező anyagcsere-termékei idézték elő. Ezt alátámasztják a Prymnesium parvum szervezetet tartalmazó vízminta víz-toxikológiai eredményei, valamint a specifikus hemolitikus-teszt értékei. A vízvirágzást előidéző szervezet kivonatából 15 jól definiálható proteáz aktivitású fehérjét mutattunk ki 8-as pH-n (pH optimum). Összevetve más algák, illetve cianobaktériumok proteáz-mintázatával, ez a szám kifejezetten nagynak mondható. Irodalom Collins, M.: (1978) Algal toxins. Microbiol. Rev. 42, 725-746. 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Toxicity of the extracellular phase of Prymnesium parvum cultures. J. Gen. Microbiol. 24, 165. Abstract: The haptophyte alga Prymnesium parvum has been responsible for toxic incidents with severe ecological impact in many parts of the world. This organism produces a set of highly potent exotoxins commonly called prymnesins. These toxins have hannful biological effects, including ichthyotoxic, neurotoxic, cytotoxic, hepatotoxic and hemolytic activity towards a range of organisms in marine brackish- and freshwater. Gill breathing animals such as fish and molluscs (e.g. bivalves) are especially vulnerable as prymnesins destroy the gill tissue. This study documents a relationship between a Prymnesium parvum bloom and incident of fish-destruction in a Hungarian pond near Hajdúszoboszló. Toxicity of the P. parvum was quantified by measuring the haemolytic activity of water samples and by the influences of cell-free filtrates of P. parvum , harvested from waterbloom sample, in the toxicological test of fish and daphnia. We have described for the first time the presence of high amount of proteases in a water sample containing enormous Prymnesium parvum population, that could be essential for understanding the killing mechanism of this organism. Keywords: Prymnesium parvum , water-bloom, ichtyotoxic, protease. Klossy Irén alkotása (Fametszet, Magyar tájak sorozat)
