Fogorvosi szemle, 2017 (110. évfolyam, 1-4. szám)
2017-09-01 / 3. szám
87 FOGORVOSI SZEMLE ■ 110. évf. 3. sz. 2017. 11. Luo Y, Kirker KR, Prestwich GD: Cross-linked hyaluronic acid hydrogel films: new biomaterials for drug delivery. J Control Release. 2000; 69: 169-184. 12. Park SJ, Li Z, Hwang IN, Huh KM, Min K-S: Glycol Chitin— based Thermoresponsive Hydrogel Scaffold Supplemented with Enamel Matrix Derivative Promotes Odontogenic Differentiation of Human Dental Pulp Cells. J Endod. 2013; 39: 1001 — 1007. 13. PRESTON M, SHERMAN LS: Neural Stem Cell Niches: Critical Roles for the Hyaluronan-Based Extracellular Matrix in Neural Stem Cell Proliferation and Differentiation. Front Biosci (Sehol Ed). 2011; 3: 1165-1179. 14. Prestwich GD: Engineering a clinically-useful matrix for cell therapy. Organogenesis. 2008; 4, 42-47. 15. Segura T, Anderson BC, Chung PH, Webber RE, Shull KR, Shea LD: Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern. Biomaterials. 2005; 26: 359-371. 16. Shu Xz, Liu Y, Luo Y, Roberts Me, Prestwich Gd: Disulfide crosslinked hyaluronan hydrogels. Biomacromolecules. 2002; 3: 1304- 1311. 17. Stevens MM, George HJ: Exploring and Engineering the Cell Surface Interface. Science. 2005; 310: 1135-1138. 18. Suzuki T, Lee CH, Chen M, Zhao W, Fu SY, Qi JJ: Induced Migration of Dental Pulp Stem Cells for in vivo Pulp Regeneration. J Dent Res. 2011 ; 90: 1013-1018. 19. Todd RH, Daniel SK: Hydrogels in drug delivery: Progress and challenges. Polymer. 2008; 49: 8. 20. Toh WS, Spector M, Lee EH, Cao T: Biomaterial-Mediated Delivery of Microenvironmental Cues for Repair and Regeneration of Articular Cartilage. Mol Pharm. 2011 ; 8: 994-1001. 21. TOOLE BP: Hyaluronan: from extracellular glue to pericellular cue. Nat Rev Cancer. 2004; 4: 528-539. 22. Varga I, Sóczó G, Kardos G, Kemény-Beke A, Kelentey B, Márton I és mtsai. Differences in killing activity of caspofungin and paradoxical growth between C. albicans and C. krusei clinical isolates in different media. J Chemother. 2009; 36-41. 23. Varga I, Sóczó G, Kardos G, Borbély A, Szabó Z, Kemény-Beke A: Comparison of killing activity of caspofungin against Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis. J Antimicrob Chemother. 2008; 62: 1466-1468. 24. Varga I, Sóczó G, Kardos G, Majoros b: Time-kill studies investigating the killing activity of caspofungin against Candida dubliniensis: comparing RPMI-1640 and antibiotic medium 3. J Antimicrob Chemother. 2008; 62: 149-152. Rente T, Bakó J, Bágyi K, Jenei K and Hegedűs Cs Preparation and drug profile analysis of crosslinked hydrogels based on hyaluronic acid for dental application Exploiting the biodegradability and biocompatibility of hyaluronic acid, the hyaluronic acid-based systems are increasingly used in dentistry also. To achieve the targeted drug delivery one of the most widely used method of cross-linking the biopolymers. The purpose of this work was to produce and characterizes hyaluronic acid-based systems, which can also be used in dentistry as drug delivery system. To synthesize the different ratios (25, 50, 75, 100%) cross-linked structure hyaluronic acid sodium salt and 2,2’(ethylenedioxy) bis (ethylamine) was applied in a condensation reaction using carbodiimide. The sizes of the particles were determined by dynamic light scattering (DLS) measurements. On the basis of the results obtained that each of four kinds of modified material are made up three different particle sizes and when the rate of change was increased, the particle size was decreased. This could be compared to results obtained under transmission electron microscope (TEM) investigations. To characterize the release behavior of cross-linked derivatives of hyaluronic acid, photometric measurement technique was applied using methylene blue as a model compound. Our results supported that the proportion of cross-linking was increased, the drug release rate was increased also, which may be the basis for further experiments in the development of a new injectable drug substance issuer system. Keywords: hyaluronic acid, hydrogel, drug delivery, crosslinking, biocompatibility
