Szemészet, 1987 (124. évfolyam, 1-4. szám)

1987-02-01 / 1. szám

Eredményeik szerint az egyes enzimek szolubilis fehérjére vonatkoztatott és totál aktivitása nem változott mindig paralel a szürkehályog kialakulása során. A különbségek az egyes enzimek eltérő ellenállásából adódnak a denaturációval szemben.

IRODALOM: 1. Anderson, E. 1., Spector, A.: Exp. Eye Res. 26, 407 (1978). — 2. Barsacchi, B., Pelosi, G., Camici, P., Bonaldo, L., Maiorino, M., Ursini, F.: Biochim. Biophys. Acta 804, 356 (1984). -— 3. Bergad, P. L., Bathbun, W. B., Linder, TL.: Exp. Eye Res. 34, 131 (1982). — 4. Boyne, A. F., Ellman, G. L.: Anal. Biochem. 46, 639 (1972). — 5. Dillon, J., Spector, A., Nalcanashi, K.: Nature 259, 422 (1976). — 6. Facskó A., Édes I.: Szemészet 117, 79 (1980). — 7. Facskó A., Édes I.: Szemészet 120, 69 (1983). — 8. Fecondo, J. V., Augusteyn, R. G.: Exp. Eye Res. 36, 15 (1983). — 9. Frank, L., Massaro, D. : Am. J. Med. 69, 117 (1980). — 10. Habig, W. H., Pabst, M. J., Jakoby, W. B.: J. Biol. Chem. 249, 7130 (1974). — 11. Harding, J. J.: Biochem. J. 117, 957 (1970). — 12. Peterson, G. L.: Anal. Biochem. 83, 346 (1977). — 13. Pirié, A. : Invest. Ophthalmol. 7, 634 (1968). — 14. Bathbun, W. B., Sethna, S. S., Skelnik, D. L., Bistner, S. I.: Exp. Eye Res. 36, 845 (1983). — 15. Saneto, B. S., Awasthi, Y. C., Srivastava, S. T.: Exp. Eye Res. 34, 107 (1982). — 16. Stacey, N., Priestly, B. G.: Toxicol. Appl. Pharmacol. 45, 41 (1978). — 17. Truscott, R. J. W., Augusteyn, B. C.: Exp. Eye Res. 25, 139 (1977). — 18. Truscott, R. J. W., Augusteyn, B. C.: Biophys. Acta 492, 43 (1977).

A. a i k o, M. Egem: H3yneHue oOMehta lAtomamuoHa e HopMaAbHOM xpycmanuKe u xpycmajiuKe c Kamapaicmou

Abtop n3yMajTH H3MeHeHHH oöiweHa rjnoTaTHOHä B HopiwajibHOM xpycTajiHKe h b ycnobhhx nporpeccupoBaHHH CTapnecKOH KaTapaKTbi. fljiH xapaKTepucTHKH oŐMeHa ohh HcnoJib30BajiH cjieayioiuHe 3h3hmm: rjnoTaTHOH-peayKTa3y (QSSQ-R), rjnoTaTuoH-nepoK- cwaa3y (OSH-P) h rinoTaTH0H-S-TpaHC({)epa3y (QSH-S-T).

Kau noKa3ajin pe3yjibTaTbi HccJieaoBaHHii, aKTHBHocTb rjnoTaTH0H-peayKTa3bi aocto- BepHO B03pacTajia b HaMajibHOM CTaflHH pa3BnTHH cTap^ecKoi^ KaTapaKTbi, mto, BepoaTHO, mo>kho paccMaTpHBaTb Kax KOMneHcaTopHoe H3MeHeHHe. B xpycTaJiHKax c jjaaeKO 3ameflineii KaTapaKToii (no KJiaccn(})HKauHH Pirié III n IV rpynnbi) aKTHBHOCTb Bcex Hccneaye- Mbix 3H3HM0B (xaK GSSG-R, Tax GSH-P n GSH-S-T) CTaTHCTnaecKH aocTOBepHO chh3hjiacb cpaBHHTejibHO c KOHTpojieM.

CornacHO noJiyneHHbiM pe3yjibTaTaM, TOTajibHaa aKTHBHocTb oTaeJibHbix 3H3hmob h OTHeceHHaa k pacTBopnMOMy 6enKy He Bceraa H3MeHajmcb napajureabHO b xone bo3hhkho- BeHHH KaTapaKTbi. Pa3JlHHHa B03HHKaiOT BCJieaCTBHe pa3H0ii yCTOHHHBOCTH OT/jeJTbHbIX 3H3HM0B k aeHaTypauHH.

Facskó A., Édes I.: Examination of glutation metabolism in normal and cataractous lenses

Changes of glutation metabolism were examined in normal and in senile cataractous lenses. For a characterization of metabolism the following enzymes were used : glutation reductase (GSSG-R), glutation peroxydase (GSH-P) and glutation-S-transferase (GSHS-T).

According to results in the early stage of senile lens opacification the GSSG-R activity is raising significantly which may be regarded as a compensatory mechanism. In progressed stage of cataract development (according to Pirié the III. and IV. group) the GSSG-R as well as GSH-P and GSH-S-T activity decreased significantly in comparison with normal controls.

According to the results the total activity of certain proteins and their correlation with soluble proteins changed not always parallel with the development of cataract. The disparity may be rise from a different resistance of certain proteins to denaturation.

A. Facskó und I. Édes: Untersuchung des Glutathionmetabolismus in normalen und kataraktischen Linsen

In normalen Linsen und Linsen mit fortgeschrittenem Altersstar wurden die Änderungen des Glutationstoffwechsels untersucht. Zur Charakterisierung des Stoffwechsels dienten folgende Enzyme: Glutathion-Reduktase (GSSG-R), Glutathion-Peroxidase (GSH-P) und Glutathion-S-Transferase (GSH-S-T).

Die GSSG-R-Aktivität hat sich im inzipienten Stadium des Altersstars signifikant erhöht, was wahrscheinlich als eine kompensatorische Veränderung zu betrachten ist.

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