202280. lajstromszámú szabadalom • Eljárás funkcionális humán urokináz fehérjék előállítására
HU 202280 B Int Cl5 C 12 N 15/58 C 12 N 15/70 C 12 N 15/85 AccI BstNI Mn 781 CATCGTCTACCTGGGTCGCTCAAGGCTTAACTCCAACACGCAAGGGGAGATGAAGTTTGA GTAGCAGATGGACCCAGCGAGTTCCGAATTGAGGTTGTGCGTTCCCCTCTACTTCAAACT EcoRII II Mn1I Haell Hphl 841 GGTGGAAAACCTCATCCTACACAAGGACTACAGCGCTGACACGCTTGCTCACCACAACGA CCACCTTTTGGAGTAGGATGTGTTCCTGATGTCGCGACTGTGCGAACGAGTGGTGTTGCT Hhal MbolISau3A Mnll MstIFnu4HI Ncil 901 CATTGCCTTGCTGAAGATCCGTTCCAAGGAGGGCAGGTGTGCGCAGCCATCCCGGACTAT GTAACGGAACGACTTCTAGGCAAGGTTCCTCCCGTCCACACGCGTCGGTAGGGCCTGATA XholIOpnl HhaIBbv Hpall Mill Sau3A Alul Opn 961 ACAGACCATCTGCCTGCCCTCGATGTATAACGATCCCCAGTTTGGCACAAGCTGTGAGAT TGTCTGGTAGACGGACGGGAGCTACATATTGCTAGGGGTCAAACCGTGTTCGACACTCTA Taql DpnI Sau I EcoRI HpaIIFnu4HIPvuII 1021 CACTGGCTTTGGAAAAGAGAATTCTACCGACTATCTCTATCCGGAGCAGCTGAAAATGAC GTGACCGAAACCTTTTCTCTTAAGATGGCTGATAGAGATAGGCCTCGTCGACTTTTACTG 3A BbvAluI Alul Hpall Bbv H 1081 TGTTGTGAAGCTGATTTCCCACCGGGAGTGTCAGCAGCCCCACTACTACGGCTCTGAAGT ACAACACTTCGACTAAAGGGTGGCCCTCACAGTCGTCGGGGTGATGATGCCGAGACTTCA Ncil Fnu4HI phl Bbv HinfI EcoRII Hi 1141 CAC CAC CAAAATGCTATGTGCTGCTGAC CCCCAATGGAAAACAGATTCCTGCCAGGGAGA GTGGTGGTTTTACGATACACGACGACTGGGGGTTACCTTTTGTCTAAGGACGGTCCCTCT Fnu4Hl BstNI nfIDdelAvalIMnlI Mnll HaellI A1 1201 CTCAGGGGGACCCCTCGTCTGTTCCCTCCAAGGCCGCATGACTTTGACTGGAATTGTGAG GAGTCCCCCTGGGGAGCAGACAAGGGAGGTTCCGGCGTACTGAAACTGACCTTAACACTC Sau96 Fnu4HI ul Sau96 EcoRIIAcylAccI HinfI 1261 CTGGGGCCGTGGATGTGCCCTGAAGGACAAGCCAGGCGTCTACACGAGAGTCTCACACTT GACCCCGGCACCTACACGGGACTTCCTGTTCGGTCCGCAGATGTGCTCTCAGAGTGTGAA HaelII BstNlHgal BstNIBamHIDpnI FboII HaelBstNIHaellIMnl IMnl IS 1321 CTTAC CCT GGATCCGCAGT CACACCAAGGAAGAGAAT GGCCTGGCCCTCTGAGGGTCCCC GAATGGGACCTAGGCGTCAGTGTGGTTCCTTCTCTTACCGGACCGGGAGACTCCCAGGGG EcoRIIXhoIISau3A HaelIIEcoRIISau96DdeIAv BstNIMnlI HinfI 1381 AGGGAGGAAACGGGCACCACCCGCTTTCTTGCTGGTTGTCATTTTTGCAGTAGAGTCATC TCCCTCCTTTGCCCGTGGTGGGCGAAAGAACGACCAACAGTAAAAACGTCATCTCAGTAG EcoRI I 1441 1501 PvuII MboII MboII TCCATCAGCTGTAAGAAGAGACTGGGAAGATAGGCTCTGCACAGATGGATTTGCCTGTGG AGGTAGTCGACATTCTTCTCTGACCCTTCTATCCGAGACGTGTCTACCTAAACGGACACC Alul EcoRIIHphl Alul Mnll HaeIHaeIIIEcoRIIFnu4 CACCACCAGGGTGAACGACAATAGCTTTACCCTCACGGATAGGCCTGGGTGCTGGCTGCC GTGGTGGTCCCACTTGCTGTTATCGAAATGGGAGTGCCTATCCGGACCCACGACCGACGG BstNI StuIBstNl Bbv 3B. ábra
