200795. lajstromszámú szabadalom • Eljárás többszörös horgokat tartalmazó plazminogén aktivátorok előállítására
HU 200795 B Int Cl5 C 12 N 15/58 A 61 K 37/54 Az 1/a ábra szerinti hármas-horog PA 1 11 21 31 41 51 GGATCCTCGACATGAGAGCCCTGCTGGCGCGCCTGCTTCTCTGCGTCCTGGTCGTGAGCG MetArgAlaLeuLeuAlaArgleuLeuLeuCysValLeuValValSerAsp 61 71 81 91 101 111 ACTCCAAAGGCAGCAATGAACTTCATCAAGTTCCATCGAACTGTGACTGTCTAAATGGAG SerLysGlySerAsnGluleuHisGlnValProSerAsnCysAspCysLeuAsnGlyGly r-------- I----------------------------------------------------------------------------------121 131 141 151 161 171 GAACATGTGTGTCCAACAAGTACTTCTCCAACATTCACTGGTGCAACTGCCCAAAGAAAT ThrCysValSerAsnLysTyrPheSerAsnIleHisTrpCysAsnCysProLy5lysPhe 181 191 201 211 221 231 TCGGAGGGCAGCACTGTGAAAT AGAT AAGT CAAAAACCTGCT ATGAGGGGAATGGTCACT GlyGlyGlnHisCysGluIleAspLysSerLysThrCysTyrGluGlyAsnGlyHisPhe 241 251 261 271 281 291 TTTACCGAGGAAAGGCCAGCACTGACACCATGGGCCGGCCCTGCCTGCCCTGGAACTCTG TyrArgGlyLysAlaSerThrAspThiWetGlyArgProCysLeuProTrpAsnSerAla 301 311 321 331 341 351 CCACTGTCCTTCAGCAAACGTACCATGCCCACAGATCTGATGCTCTTCAGCTGGGCCTGG ThrValLeuGlnGlnThrTyrHisAlaHisArgSerAspAlaLeuGlnleuGlyLeuGly 361 371 381 391 401 411 GGAAACATAATTACTGCAGGAACCCAGACAACCGGAGCCGACCCTGGTGCTATGTGCAGG LysHisAsnTyrCysArgAsnProAspAsnArgArgArgProTrpCysTyrValGlnVal 421 431 441 451 461 471 TGGGCCTAAAGCCGCTTGTCCAAGAGTGCATGGTGCATGACTGCAGCGAGGGCAACTCCG , GlyLeuLysProLeuValGlnGluCysMetValHisAspCysSerGluGlyAsnSerAsp 481 491 501 511 521 531 ACTGCTACGAGGACCAGGGCATCAGCTACAGGGGCACGTGGAGCACAGCGGAGAGTGGCG CysTyrGluAspGlnGlyIle5erTyrArgGlyThrTrpSerThrAlaGluSerGlyAla 541 551 561 571 581 591 CCGAGTGCACCAACTGGAACAGCAGCGCGTTGGCCCAGAAGCCCTACAGCGGGCGGAGGC GluCysThrAsnT rpAsnSerSerAlaleuAlaGlnLysProT yrSerGlyArgArgPro 601 611 621 631 641 651 CAGACGCCATCAGGCTGGGCCTGGGGAACCACAACTACTGCAGAAACCCAGATCGAGACT AspAlalleArgLeuGlyLeuGlyAsnHisAsnTyrCysArgAsnProAspArgAspSer 661 671 681 691 701 711 CAAAGCCCTGGTGCTACGTCTTTAAGGCGGGGAAGTACAGCTCAGAGTTCTGCAGCACCC LysProTrpCysTyrValPheLysAlaGlyLysTyrSerSerGluPheCysSerThrPro 721 731 741 751 761 771 CTGCCTGCTCTGAGGGAAACAGTGACTGCTACTTTGGGAATGGGTCAGCCTACCGTGGCA AlaCysSerGluGlyAsnSerAspCysTyrPheGlyAsnGlySerAlaTyrArgGlyThr 781 791 801 811 821 831 CGCACAGCCTCACCGAGTCGGGTGCCTCCTGCCTCCCGTGGAATTCCATGATCCTGATAG HisSerleuThrGluSerGlyAlaSerCysLeuProTrpAsnSerMetlleLeuIleGly 841 851 861 871 881 891 GCAAGGTTTACACAGCACAGAACCCCAGTGCCCAGCCACTGGGCCTGGGCAAACATAATT p LysValTyrThrAlaGlnAsnProSerAlaGlnAlal.euGlyLeuGlyLysHisAsnTyr UK szignál peptid, 20 aminosav UK szekvencia (aal-131) hexapeptid kapcsoló 9. ábra
