Dr. Holló Ferenc - Dr. Murai Éva szerk.: Parasitologia Hungarica 5. (Budapest, 1972)
Caljtcophoron calicophorum and Paramphistomum gotoi by YAMAGUTI /1940/, those of P. explanatum and P. orthocoelium by OZAKI /1951/, of P./= Liorchis/ hiberniae by WILLMOTT /1952/ and KATKOV /1969/, and the embryonal growth of P. gotoi by TANDON /1960/ and STEPANOV /1969/. The European occurrence of Paramphistomum daubneyi was established for the first time by the present author ; a detailed description of this species can be found in DINNIK's paper /1^62/. Its growth, ecological and biological requirements under home conditions are not known. The aim of the present paper is the biometrical and morphological investigation of the eggs of P. daubneyi , the study of the embryo-formation process, and of the growth and structure of the- miracidium. Material and Methods Helminths, obtained from cattle in the slaughter-house, oviposited separately in isotonic NaCl solution for some hours. After a thorough rinse, the eggs were incubated in distilled water at 27 °C in thermostat. Water was not changed on the cultures, only the evaporated quantity replaced. The development of the embryo and changes in the eggs were daily checked under microscope. For the study of the eggshell's structure a polarization microscope, Laboval type /Carl Zeiss, Jena/, was used. In comparing egg and fluke sizes, the living specimens were fixed in CARNOY in every case. The glycogen contents of the vitelline cells were demonstrated by PAS reaction and the nuclei stained by haematoxyline . The hatching process of the miracidia was daily observed /from *The Paramphistomid species of cattle and sheep will be publis hed in a following paper.