Dr. Holló Ferenc - Dr. Murai Éva szerk.: Parasitologia Hungarica 5. (Budapest, 1972)

Caljtcophoron calicophorum and Paramphistomum gotoi by YAMAGUTI /1940/, those of P. explanatum and P. orthocoelium by OZAKI /1951/, of P./= Liorchis/ hiberniae by WILLMOTT /1952/ and KAT­KOV /1969/, and the embryonal growth of P. gotoi by TANDON /1960/ and STEPANOV /1969/. The European occurrence of Paramphistomum daubneyi was estab­lished for the first time by the present author ; a detailed description of this species can be found in DINNIK's paper /1^62/. Its growth, ecological and biological requirements un­der home conditions are not known. The aim of the present paper is the biometrical and morphologi­cal investigation of the eggs of P. daubneyi , the study of the embryo-formation process, and of the growth and structure of the- miracidium. Material and Methods Helminths, obtained from cattle in the slaughter-house, ovipo­sited separately in isotonic NaCl solution for some hours. Af­ter a thorough rinse, the eggs were incubated in distilled wa­ter at 27 °C in thermostat. Water was not changed on the cultu­res, only the evaporated quantity replaced. The development of the embryo and changes in the eggs were daily checked under microscope. For the study of the eggshell's structure a polari­zation microscope, Laboval type /Carl Zeiss, Jena/, was used. In comparing egg and fluke sizes, the living specimens were fixed in CARNOY in every case. The glycogen contents of the vi­telline cells were demonstrated by PAS reaction and the nuclei stained by haematoxyline . The hatching process of the miracidia was daily observed /from *The Paramphistomid species of cattle and sheep will be publis hed in a following paper.

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