Dr. Holló Ferenc szerk.: Parasitologia Hungarica 3. (Budapest, 1970)
gyloides contain the first larva when discharged in the faeces. Some incidentally, detected eggs of free-living (non-parasitic) soil-, and plant-nematodes may morphologically resemble those of Trichostrongylidae . For a specific diagnosis larvae were also cultured especially as only once previously had Tricho strongylus infection in man been diagnosed in this country by identification of the infective larvae (VÉGHELYI et al., 1948). Stool culture : The method of culturing larvae has been employed for some time in veterinary parasitology (HOLLÓ, 1969) but it is still a less familiar method for diagnosing human parasitic infections. It is to some extent similar to the method for culturing Ancylostoma larvae (lENGYEL et al., 1968). Our method, recommended for use in other laboratories, is as follows. Faeces of pulpy consistency are smeared in a thin layer on a round filter-paper which is placed on a moist sponge in a Petri dish. The permanently moist faecal culture is kept at a temperature of 28-30° C for 6-8 days. The larvae, which have developed to the third stage in the meantime, are separated by the principle of BAERMANN ' s technique,as follows: the filter-paper, with the faecal smear inverted is laid on a sieve fitted into a funnel filled with tap-water of 37° C up to the bottom of the sieve (i.e. to the level of the faecal smear). The larvae, because of their positive hydro- and thermo-taxis , migrate into the water and settle at the bottom of the funnel just above the tap, and may be removed to centrifuge tubes after about 1 hour. They are then spun down for 3 minutes and the sediment examined under the microscope. Alternatively, larvae can be isolated in a conical flask (NEMESÉRI and HOLLÓ, 1961). The sieve, containing the faecal smear on filter paper, is placed on the top of the flask which is filled with warm water to the level of the faeces.The larvae ,having sedimented to the bottom of the flask, are pipetted on to a slide, and their size and behaviour examined, using x 20 magnification. Differential diagnosis : By morphological features the third
