Matskási István (szerk.): A Magyar Természettudományi Múzeum évkönyve 88. (Budapest 1996)

Szedlay, Gy., Jakucs, E. , Bóka, K. ; Boldizsár, I.: Macro- and micromorphological characteristics of Ganoderma lucidum Karsten strains isolated in Hungary

size was measured by the average of about ten basidiospores. Pilocystidia were prepared by diluting the laccate layer by placing a small piece of the surface tissue from the pileus into acetone for 24, 48 and 72 hours and coated. Optimal growth temperature Optimal growth temperature of the strains was determined on malt agar (MEA) and potato dextrose agar (PDA) growing for 7 days at 14, 18, 22, 26, 30, 34 and 38 °C. The experiment was repeated twice in three replications. RESULTS Morphology of the fruitbodies The fruitbodies of our Ganoderma lucidum isolates were stipitate. Each pileus was more or less imbricate. The colour of the pileus surface was dark red and laccate. The young fungus had a non-laccate yellow to white margin. At the beginning the context colour was pale buff, later dark brown. The stipes was excentric, woody, with different length. The thickness of the context ranged from 5 to 30 mm. Temperature studies Temperature studies showed different optimal temperatures on MEA and PDA (Fig. 1, Table 2). In most cases growth of our strains on 14 °C was similar to that on 30 °C, mainly about the third or half of the optimal growth. Considerable growth on 14 °C had been proved also by ADASKAVEG & GiLBERTSON (1986), but WANG & HUA (1991) had demonstrated slight growth in only one case under such conditions. Although according to the data by ADASKAVEG & GILBERTSON (1986) and WANG & HUA (1991) growth on 34 and 38 °C is vigorous, none of our strains grew at all at these temperatures. As to the investigations of ADASKAVEG & GILBERTSON (1986) G. lucidum can grow in an interval of greater than 30 °C, according to WANG & HUA (1991) in an interval of 25 °C, and our Fi«. 1. The average growth curve of the one-week-old mycelial cultures grown on PDA and MEA