Horváth Géza (szerk.): A Magyar Természettudományi Múzeum évkönyve 8. (Budapest 1910)

Soós, L.: A Helix arbustorum hím csirasejtjének fejlődése

SPERMIOGENESE OF HELIX ÁBEL-STOPOM. AL)D frequently HEIDENHAIN'S iron hematoxylin, either used alone, or preceded by Bordeaux B or 0-25% anilin blue. BENDA'S crystal violet and sodiumsulplializarinate-toluidin blue have also been excellent, particularly in staining the centrosome. I have also used BIONDI-EHRLICH'S triacid. saffranin, DELAFIELD'S and BÖHMER'S hematoxylins. I. The spermatogonia. The spermatogonia of Helix

, as shown by ANCEL (2), develop from the «large germ-cells». These latter in the genital gland of the adult Helix are no more to be found. Plate VII., Fig. 1. shows the cross section of a young follicle of adult Helix arbustorum filled with ovoidal or spherical cell nuclei. Their chromatin consists either of separated clots or of small chromomeres which are connected by delicate chromatin threads. The chromomeres form in places larger masses. The nuclei have no nucleoli, and are arranged in the young latest developed follicles in one layer along the wall of the follicle, and they fill only later its whole lumen. The nuclei are imbedded in a delicate alveolar ground substance, therefore such a follicle is really a large syncytium. These nuclei represent the spermatogonia. There are to be found spread among them some peculiar cells which are originally very similar to the spermatogonia, but are later very easily distinguishable by their very large nucleus filled with rough chromatin clumps, and by their small amount of cytoplasm. These cells are the base-cells which later become the nurse-cells of the developing spermatozoa and are equivalent to the SERToLi-cells of the testes of Mammals. Spermatogonia like those described above are also to be found along the walls of those follicles in which the spermatozoa are already developing. The nuclei in these follicles are sometimes crowded so much that their outlines become indefinable, and can scarcely be distinguished. They have very often the structure shown on Fig. 2., Pl. VII, when the chromatin is divided into some clumps irregular in shape, connected with one another by delicate threads. The clumps are sometimes fairly far from one another (Pl. VII., Fig. 2.), at other times closely together, and nearly coalesced. The outlines of the cells cannot bo distinguished, because they are imbedded in a common ground plasm, as above. In Rtludinn

, as is shown by MEVES (72) the walls of the follicles are covered by giant cells, the base-cells, an the spermatogonia are imbedded in their cytoplasm. So it is, according to the same author, in Helix. But this latter statement is undoubtedly erroAnnaten Musei Maiionatia Hungarici Vlll. '