Marisia - Maros Megyei Múzeum Évkönyve 36/1. (2016)

Botany

In vitro assay of antibacterial activity of three medicinal plants

T80+ UV/VIS Spectrophotometer (PG-Instruments). Each experiment has been performed in triplicate.

Identification and separation of the major compounds of hydroethanolic

extract of herbs by TLC (thin layer chromatography) method

The standard compounds with antibacterial activity (ferulic acid, gallic acid, chlorigenic acid, quercetin, rutin, kaempferol) were purchased from the Bucharest Chemical Company. We used a semiautomatic applicator (Linóm 5 - CAMagic, Muttenz, Switzerland). The spraying was achieved using a plate spray device (Merck). Reading of the plates was performed using a device for TLC imaging (Digistore 2 - CAMagic), and the images were stored as JPEG files, without compression, to avoid losing image quality. The advantage of this method is that the detection by natural fluorescence or fluorescence quenching does not modify or destroy the compounds.

Results and discussions

From the lot of bacterial strains isolated from the kidney infection of hospitalized patients, Klebsiella pneumoniae, Acinetobacter baumanii and Proteus mirabilis strains have submitted the highest resistance to the antibiotics tested (Table 1). These strains were represented a greater interest in the analysis of antibacterial potency of the hydoethanolic extracts herbs.

Table 1: Antibiotic resistances of Gram negative bacteria

Strain Antibiotic (R=resistance; S=susceptibility)

~AMC CAZ FEP CIP CM IMI ERT STX TZP CL MEM P mirabilisn R R

R

R

R

R R

R

R

R

R

A. baumannif R R

R

R

R

R R

R

R

R

R

P aeruginosalu R R

R

R

R

R RRRS R

K. pneumoniae^ R R

R

R

R

R RRRS R

K. pneumoniae|15 R R

R

R

R

R RRRS R

A. baumanniim R R

R

R

R

R RRRS R

P. aeruginosa[1A R R

R

R

R

R RRRS R

A. baumanniim R R

R

R

R

R R

R

R

R

R

P. aeruginosaUU2 R R

R

R

R

R R

R

R

R

R

P. aeruginosanm R R

R

R

R

R RRRS R

(abbreviations: AMC - amoxicillin-clavulanic acid; CAZ - ceftazidime; FEP - cefepime; CIP- ciprofloxacin; CM- gentamicin; IPM - imipenem; ERT - ertopenem; SXT - sulfamethoxazole; TZP - piperacillin-tazobactam; CL

— colistin MEM — meropenem;)

The antioxidant activity and total content of phenols and flavonoids are reflected in figure 1 being calculated according to the protocol submitted. Following the analysis of the results, E. caryophyllata had the highest antioxidant activity (expressed in mg ascorbic acid / g plant) and the highest polyphenol content (mg gallic acid equivalent / g plant) compared to the other plants. Instead, the content of flavonoids (mg rutin mg / g plant) had the lowest value.

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