Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

Investigated bone samples and methods

Autosomal STR-marker frequencies:

The allele frequencies are the Hungarian data from the A11STR Autosomal Database (http://allstr.de/allstr/home.seam

)

with the exception of the D9S1120 marker data, which are from the article of Phillips et

al.

(2008), and the SE33 marker data, which are from the book of Butler (2015) (the data gathered are stored in a separate Excel file).

Y-STR frequencies and rate of mutation:

International Society of Genetic Genealogy (ISOGG) database, from the chapter “Y-DNA STR frequencies”. HTTP://ISOGG.ORG/

WIKI/Y-STR

Y-STR mutation database: http://isogg.org/wiki/Mutation_rates

Subchapter: A table of mutation rates for 111 markers provided by Marko Heinila

Low molecular weight DNA (<300bps) enrichment from all samples was performed using AMPure XP beads (NEB). Library preparation was performed using the TruSeq Nano DNA LT kit (Illumina) according to the manufacturers recommendations. Library size and quality was confirmed with Fragment Analyzer (Advanced Analytical) and quantitative PCR (Biorad SI000; CFX96 Real Time System). Paired-end sequencing (2X125 bps; 250 Cycles) was performed on the Illumina HiSeq 2500 System (Illumina) using high output flow cells in multiple runs following the manufacturer s recommendations.

232