Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

Investigated bone samples and methods

opening the Vac Valves. The MinElutes were inserted into collection tubes and centrifuged for 1 min at 15,700 ref and then placed at room temperature with open lids for 20 min to remove any remaining ethanol from the PE buffer. For elution of the DNA 20 pl of warm RNase free water (Qiagen) were pipetted to the membranes and after waiting for 5 min the columns were centrifuged for 1 min at 15,700 ref. The elution step was repeated three times for each sample. The extracts were stored at -20°C.

Qi a Vac MinElute Short

The duration of the EDTA/Proteinase K incubation was 1 h. Subsequently, a further 50 pl of Proteinase K was added and the samples were rotated for another hour at 56°C. The lysate was centrifuged for 3 min at 3,300 ref.

The supernatant was purified on MinElute columns with large volume funnels using a QIAvac 24 Plus vacuum system (see above). The extracts were stored at -20°C.

QiaVAc MinElute Organic

The duration of the EDTA/Proteinase K incubation was 18 h. Subsequently, a further 50 pl of Proteinase K was added and the samples were rotated for 1 h at 56°C. The lysate was centrifuged for 3 min at 3,300 ref.

The supernatant was mixed with 3 ml Phenol by inverting for 6 min. For phase separation the samples were placed for 10 min at 56°C. The organic phase was removed, and the samples were mixed with 4.5 ml chloroform by inverting for 6 min. Again, the phases were separated as described above. The aqueous phase was purified 227