Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)
CHAPTER EIGHT – PCR and NGS investigations
Al allele we obtained from M4 and the Al allele of skeleton 11/52 were completely identical. The above difficulties in detection may have been due to other reasons besides DNA fragmentation, since in the area marked by an arrow on the figure, sequence variation can be seen, which is equivalent to A>G transition on the reverse strand, and T>C transition on the forward strand, which influenced hybridization of the PCR primer. We know that DNA sequence polymorphism can occur within or in nearby repeating sequences. If the base swap is in accordance with the primer binding site, as in this case, then hybridization of the primer cannot occur or only occurs at a lower hybridization temperature, and thus the marker on the template will not be detectable. This was probably the situation that rendered detection of the D7S818 marker via PCR and NGS technologies more difficult and was responsible for the formation of the PCR artifact (Table 11, Figure 38). Despite the above technical difficulty, the bone samples of skeleton 11/52 and Béla III are identical in terms of the 8-bp allele length. 161
