Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

CHAPTER EIGHT – PCR and NGS investigations

Table 13. Repeating units consisting of 4 bases in square brackets, and the number of repetitions detected per allele. A mutated tetramer unit became inserted between the repeating units in markers D2S1334, D2S1334 and D19S443, which counts towards the allele length. The upper (5’) and lower (3’) genominal DNA sequence parts in contact with the repetitions are also shown.

2. Evaluating the sequencing data

D2S441: The PCR and NGS results for skeleton 11/52 and Béla

III

were exactly the same. Thus, in this case the NGS result confirmed the PCR result that the Al and A2 alleles of Béla

III

and skeleton 11/52 are different from each other. Due to the short allele lengths, no PCR artifact can be seen (Table 13, Figure 35).

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