Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

CHAPTER EIGHT – PCR and NGS investigations

Table 12. Comparison of the A-STR marker data generated using the PCR method and DNA sequencing in DNA samples isolated from the skeletons of Hungarian Árpád

Dynasty

kings II/52_3 and Béla

III. The bone samples selected can be found in Tables 6 and 7. The data from the Göttingen

and

Budapest-1 laboratories are displayed separately for each lab, along with the frequency of detectability.

Bl and B2 after the marker names refer to the Budapest-1 and Budapest-2 laboratory samples. We display every single piece of sequencing data as a sample, with the alleles coverage (the number of reads that detect them). Abbreviations: Al: allele 1, A2: allele 2: Ratio: the numerator shows the fingerprint allele detections number, the denominator indicates the number of total attempts. Fp. allele: fingerprint allele; Read no.: number of sequenced DNA strands (coverage).

In the course of sequencing, we obtain either a forward sequence (D2S441, D3S1358) or a reverse sequence (D2S1338, D7S820, D19S443). This is visible in the sequencing diagrams attached, but the following table shows the repeating unit with its forward sequence in all cases (Table 13).

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