Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)
CHAPTER EIGHT – PCR and NGS investigations
We used the “Short Tandem Repeat DNA Internet Database” (STRBase) operated by the US National Institute of Standards and Technology to compare STR-marker sequencing data (Butler - Reeder 1997; Ruitberg et al. 2001). The following data can be found in the database: name and alternate name, the precise chromosome location, GenBank availability, the structure of repeats, the PCR primer sequences necessary for the investigation of the regions, the allele sequences attributed to allele lengths, and the region of the repetitions expressed in basepairs (bp). The manufacturer of the kit needed for STR detection supplies a marker set (ladder) corresponding to each markers allele length, i.e. to the number of repeating units, and we compare the PCR products to these. It is important to note that in some cases the chromosome region in question has such a complicated structure that the consensus allele length defined by the ladder is not necessarily identical to the number of repeating units in a given sample. This is the situation in the case of marker D19S433. (The STRbase database notes: “nomenclature for supplied allelic ladders does not agree with repeat structure shown”.) The individual marker s chromosome location, the genome region denoting its location, and the PCR primer we used can be found in the Chapter entitled Investigation of bone samples and methods. The results are presented in Table 12. 153
