Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

CHAPTER EIGHT – PCR and NGS investigations

CHAPTER EIGHT

ERZSÉBET CSERNÁK, JÁNOS MOLNÁR, ZOLTÁN SZENTIRMAY

PCR AND

NGS INVESTIGATIONS

1. PCR and

NGS

analysis

of the chromosome regions of five selected A-STR markers

As we shall see later, the allele data for these markers are different in the case of Béla

III

and skeleton II/52_3, and this necessitated the DNA sequence analysis of the chromosome regions that belong to these markers. More specifically, we only wished to sequence the five A-STR marker regions, the alleles of which were different in the DNA samples isolated from the bones of Béla

III

and skeleton 11/52 using PCR analysis. These markers were the following: D2S441, D2S1338, D3S1258, D7S820 and D19S433. We analyzed the template DNA strands generated in the PCR-amplified chromosome region after sequence capture, followed by magnetic separation and subsequent amplicon library creation.

Tarsus-1 belonged to skeleton II/52_3, while tarsus-2 belonged to the skeleton of Béla

III.

We took two bone samples for both skeleton

151