Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

CHAPTER SEVEN – Genetic investigations

Anne of Antioch and the fetus) were quite degraded compared to the other group’s DNA samples, and thus it was much harder to detect A-STR markers (red line). Frequency of detectability in the cases of individual STR markers: number of accepted identical (fingerprint) allele lengths/ number of total attempts. The calculation was made using the Final Report-2 data from the Göttingen

laboratory.

6. Comparison of A-STR markers of the bones of skeleton II/52_3. PCR study

The purpose of the comparison was the numerical presentation of the Göttingen

laboratory

’s observations pertaining to the DNA isolated from skeleton II/52_3, but we also wished to gather data on the correspondence between the A-STR markers from the femur and tarsus-1, in order to interpret the results. In Table 7, we compare 20 A-STR markers of the femur from skeleton II/52_3 to the marker data of tarsus-1 generated by two different laboratories. At this point, we would like to note that all of the bones of skeleton II/52_3 belong to the same individual, and thus in the period following their discovery, sorting and reassembly of the mixed-up bones using anthropological methods was done without error. The laboratories in Budapest and Göttingen

both

received one of each bone sample; the boxes highlighted in yellow show the allele data from the A-STR investigation that differ from each other, suggesting a PCR error. Due to the poor bone structure of skeleton II/52_3, some marker alleles could not be detected even after several attempts (see Chapter 6, Figure 20 C and D).

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