Hidrológiai Közlöny, 2015 (95. évfolyam)
2015 / 5-6. különszám - LVI. Hidrobiológus Napok előadásai
76 HIDROLÓGIAI KÖZLÖNY 2015. 95. ÉVF. 5-6. SZ. Irodalom Baek, K., Jo, H., Choi, A., Kang, /., Cho, J.C. (2014) Ulvibacler marin- us sp. nov., isolated from coastal seawater. - Int. J. Syst. Evői. Microbiol. 64: 2041-2046. Boros, E., Horváth, Zs., Wolfram, G., Vörös, L. (2014) Salinity and ionic composition of the shallow astatic soda pans in the Carpathian Basin. - Ann. Limnol. - Int. J. Lim. 50: 59-69. Borsodi, A.K., Knäb, M., Czeibert, K., Márialigeti, K., Vörös, I... Somogyi, B. (2013) Planktonic bacterial community composition of an extremely shallow soda pond during a phytoplankton bloom revealed by cultivation and molecular cloning. - Extremophiles 17: 575-584. Brettar, I., Christen, R., Hö)le M.G. (2004) Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacte- rium-Bacteroides group isolated from surface water of the central Baltic Sea. - Int. J. Syst. Bacterial. 54: 65-70. Cardenas, E„ Tiedje, J.M. (2008) New tools for discovering and characterizing microbial diversity. - Curr. Opin. Biotechnol. 19: 544- 549. Eaton, A.D., Clesceri, L.S., Rice, E.W., Greenberg, A.E., Franson, M.A.H. (szerk) (2005) Standard methods for the examination of water and wastewater. 21 st ed. American Public Health Association, Washington, DC. Felföldi, T., Somogyi, B., Márialigeti, K., Vörös, L. (2009) Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe). - J. Limnol. 68: 385-395. Imhojf, J.F., Triiper, H.G., Pfennig, N. (1984) Rearrangement of the Species and Genera of the Phototrophic “Purple Nonsulfur Bacteria”. - Int. J. Syst. Bacteriol. 34:340-343 Kang, I., Lee, K., Yang, S-J., Choi, A., Kang, D., Lee, Y.K., Cho, J-C. (2012) Genome sequence of "Candidatus Aquiluna" sp. strain IM- CC13023, a marine member of the Actinobacteria isolated from an arctic fjord. - J. Bacteriol. 194: 3550-3551. Klindworth, A., Pruesse, E., Schweer, T, Peplies, J., Quast, C„ Horn, M., Glöckner, F.O. (2013) Evaluation of general 16S ribosomal R- NA gene PCR primers for classical and next-generation sequencing-based diversity studies. —Nucleic Acids Res. 41: el. Labrenz, M., Hirsch, P. (2005) Genus XIX. Roseovarius Labrenz, Collins, Lawson, Tindall, Schumann and Hirsch 1999, 145VP. In: Brenner, D.J., Krieg, N.R., Stanley, J.T., Garrity, G.M. (szerk.), Bergey’s manual of systematic bacteriology. 2nd ed. Vol. 2. The Proteobacteria. Part C. Springer, New York,pp. 215-217. Maclsaac E.A., Stockner J.G. (1993) Enumeration of phototrophic picoplankton by autofluorescence microscopy. In: Kemp, P.F., Sherr, B.F.,Sherr, E.B., Cole, JJ. (szerk.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, pp. 187-197. Madigan, M.T., Martinko, J.M., Stahl, D„ Clark, D.P. (2010) Brock Biology of Microorganisms (13th Ed). Pearson, San Francisco. Meyer, F„ Paarmann, D., D'Souza, M., Olson, R., Glass, E.M., Kubai, M., Paczian, T., Rodriguez, A., Stevens, R., Wilke, A., Wilkening, J., Edwards, R.A. (2008) The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes. - BMC Bioinformatics 9:386. Pruesse, E., Peplies, J., Glöckner, F.O. (2012) SINA: accurate high- throughput multiple sequence alignment of ribosomal RNA genes. - Bioinformatics 28:1823-1829. Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T, Yarza, P., Peplies, J., Glöckner, F.O. (2013) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. -Nucl. Acids Res. 41: D590-D596. Schloss, P.D., Westcott, S.L., Ryabin, T, Hall, J.R., Hartmann, M„ Hollister, E.B., Lesniewski, R.A., Oakley, B.B., Parks, D.H., Robinson, C.J., Sahl, J.W., Stres, B., Thallinger, G.G., Van Horn D.J., Weber, C.F. (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. - Appl. Environ. Microbiol. 75: 7537-7541. Segata, N, Boemigen, D., Tickle, T.L., Morgan, X.C., Garrett, W.S., Huttenhower, C. (2013) Computational meta'omics for microbial community studies. - Mol. Syst. Biol. 9: 666. Sipos, R., Székely A. J., Palatinszky M., Révész S., Márialigeti K., Niko- lausz M. (2007) Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targeting bacterial community analysis. - FEMS Microbiol. Ecol. 60: 341-350. Somogyi, B., Felföldi, T, Vanyovszki, J., Agyi, A., Márialigeti, K, Vörös, L. (2009) Winter bloom of picoeukaryotes in Hungarian shallow turbid soda pans and the role of light and temperature. - Aqu- at. Ecol. 43: 735-744. Somogyi, B., Felföldi, I, Solymosi, K., Makk, J., Homonnay, Z.G., Horváth, G., Turcsi, E., Böddi, B., Márialigeti, K., Vörös, L. (2011) Chloroparva pannonica gen. et sp. nov. (Trebouxiophyc- eae, Chlorophyta) - a new picoplanktonic green alga from a turbid, shallow soda pan. - Phycologia 50: 1-10. Sorokin, D.Y., Berben, T, Melton, E.D., Overmars, L, Vavourakis, CD., Muyzer, G. (2014) Microbial diversity and biogeochemical cycling in soda lakes. - Extremophiles. 18: 791-809. Tindall, B.J., Rosselló-Móra, R„ Busse, H.-J, Ludwig, W., Kämpfer, P. (2010) Notes on the characterization of prokaryote strains for taxonomic purposes. - Int. J. Syst. Evol. Microbiol. 60: 249-266. Wang, Y.X., Li, Y.P., Liu, J.H., Xiao, W„ Lai, Y.H., Li, Z.Y., Ding Z.G., Wen, M.L., Cui, X.L. (2013) Gracilimonas mengyeensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-western China. - Int. J. Syst. Evol. Microbiol. 63(11): 3989-93. Wellburn, A.R. (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. - J. Plant. Physiol. 144: 307- 313. Willems A., Gillis, M. (2005) Genus V. Hydrogenophaga Willems, Busse, Goor, Pot, Falsen, Jantzen, Hoste, Gillis, Kersters, Auling and De Ley 1989, 329VP. . In: Brenner, D.J., Krieg, N.R., Stanley, J.T., Garrity, G.M. (szerk.), Bergey’s anual of systematic bacteriology. 2nd ed. Vol. 2. The Proteobacteria. Part C. Springer, New York. pp. 710-716. Taxonomic and functional genomic analysis of the planktonic microbial community inhabiting an astatic soda pond Szabó, A., Korponai, K., Somogyi, B., Vörös, L., Jurecska, L., Márialigeti, K. and Felföldi, T. Abstract: Astatic soda ponds of the Carpathian Basin are light-limited due to their high suspended matter content and/or chromophoric dissolved organic matter content. This and their special chemical composition, furthermore their high nutrient content, make these habitats unique worldwide. Due to their characteristic physico-chemical properties, microbial communities living in these ponds are also unique. This study aimed to obtain a more detailed view about the structure and function of microorganisms with high-resolution genomic tools in the case of a soda pond prior desiccation, Büdös-szék pond near Szabadszállás in November 2012. For taxon identification, the V3-V4 region of the 16S rRNA gene was analyzed on a Roche GS Junior DNA sequencer. For the functional analysis, a shotgun metagenomic approach was applied on an Ion Torrent PGM genetic analyzer. Results showed a taxonomically and functionally complex microbial community structure dominated by members of the phyla Proteobacteria, Actinobacteria, and Bacteroidetes with the remarkable contribution of two uncultured Actinomicrobiaceae genera and the genera Gracilimonas, Belliella, Hydrogenophaga, Roseovarius and their relatives. Functional genomic analysis revealed various modes of adaptation to the alkaline environment and to the desiccating character of the studied habitat, which were present in the community gene pool; furthermore, the importance of the serine-pathway in the carbon cycle of this environment. Keywords: 16S rRNA gene, soda pond, metagenome, Na+/H+ antiporters, next-generation DNA sequencing, functional genomics
