Hidrológiai Közlöny, 2014 (94. évfolyam)

2014 / 4. szám - László Kristóf - Jáger Katalin - Krett Gergely - Boros Gergely - Specziár András - Borsodi Andrea: Adatok a Balatonban élő busa fajok béltartalmának baktériumközösségeiről

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The First results on the bacterial community structures of the intestinal tracts of the Asian carps

living in Lake Balaton

László, K. - Jäger, K. - Krell, G. - Boros, G. - Specziár, A. - K. Borsodi, A.

Abstract: Since the last official stocking of filter feeding Asian carps nearly 30 years ago, silver (Hypophtalmichthys molitrix) and

bighead (Hypophtalmichthys nobilis) carps and their hybrids still occur in large numbers in Lake Balaton. They are high meat yield animals and important components of fish populations by consumption of zooplankton and organic detritus. However, little information is available about their nutritional habits. In the present study bacteriological examination of fore-, mid-and hindgut-section of the intestinal contents of three carps caught in Lake Balaton in September 2011 was performed by using cultivation based and molecular biological methods. An average of 107-108 / g CFU values was determined from the intestinal contents independently of the composition of the medium used. Among the strains isolated from the silver carps and identified according to their 16S rDNA sequence analysis, members of species Aeromonas veronii, Brevundimonas vesicularis, Micrococcus endophyticus and Bacillus halmapalus were the most abundant. From the midgut section representatives of Aeromonas veronii, Micrococcus yunnanensis and Roseomonas mucosa, while in the hindgut section Acinetobacter Iwoffii, Paracoccus yeei and Lactococcus garvieae were identified frequently. Analysis of band patterns of 16S rRNA gene sequences, obtained from the examination of the carp intestinal contents by denaturing gradient gel electrophoresis, indicated that bacterial community structures of the fore- and midguts from the same fish showed higher similarities than those of hindguts. Hindgut bacterial communities derived from different individuals formed a distinct similarity' group.

Keywords: Asian carp, microbial community' of the intestinal tract, diversity, cultivation, DGGE.