Hidrológiai Közlöny 2010 (90. évfolyam)
6. szám - LI: Hidrobiológus Napok: „Új módszerek és eljárások a hidrobiológiában” Tihany, 2009. szeptember 30–október 2.
19 Csotonyi J. T., Swiderski, J., Stackebrandt ,E., Yurkov, V .V. (2008) Novel halophilic aerobic anoxygenic phototrophs from a Canadian hypersaline spring system. Extremophiles 12: 529-539. Devereux, R., Delaney, M., Widdel, F., Stahl, D. A. (1989) Natural relationships among sulfate-reducing eubacteria. J. Bacteriol. 171: 6689-6695. Dimitriu, P. A., Shukla, S. K., Conradt, J., Marquez, M. C., Ventosa, A., Maglia, A., Peyton, B. M, Pinkart, H. C, Mormile, M. R. (2005) Nitrincola lacisaponensis gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from an alkaline, saline lake. Int. J. Syst. Evol. Microbiol. 55: 2273-2278. Eiler, A., Bertilsson, S. I. (2004) Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environ. Microbiol. 6: 1228-1243. Garnova, E. S., Zhilina, T. N., Tourova, T. P., Lysenko, A. M. (2003) Anoxynatronum sibiricum gen.nov., sp. nov. alkaliphilic saccharolytic anaerobe from cellulolytic community of Nizhnee Beloe (Transbaikal region). Extremophiles 7: 213-220. Hiraishi, A., Ueda, Y (1995) Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. Int. J. Syst. Bacteriol. 45: 319-326. Jones, B. E„ Grant, W. D., Duckworth, A. W., Owenson, G. G. (1998) Microbial diversity of soda lakes. Extremophiles 2:191-200 Madigan, M. T. (2003) Anoxygenic phototrophic bacteria from extreme environments. Photosynth. Res. 76: 157-171. Mesbah, N. M., Abou-El-Ela, S. H., Wieget, J. (2007) Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Microb. Ecol. 54: 598-617. Milford, A. D., Achenbach, L. A., Jung, D. O., Madigan, M. T. (2000) Rhodobaca bogoriensis gen. nov. and sp. nov., an alkaliphilic purple nonsulfur bacterium from African Rift Valley soda lakes. Arch. Microbiol. 174: 18-27. Filogenetikai csoport Legközelebbi rokon baktériumfaj Hasonlóság Törzsek KJónok (%) száma Cyanobacteria (Phylum X) Prochlorales Prochlorococcus marinus (AFI 80967) 89 1 Nostocales Anabaena cylindríca (AF091150) 83 1 a-Proteobacteria (Phylum XII) Sphingomonadales Erythrobacter longus (AM691106) 95 4 Rhodobacterales Rhodobaca bogoriensis (AF248638) 95 4 Rhodobacterales Rhodobaca barguziensis (EF554833) 96 1 Rhodobacterales Rhodobacter capsulatus (D 16428) 97 1 Rhodobacterales Rhodovulum strictum (D 16419) 99 1 Rhodospirillales Rubritepidaflocculans (AF465832) 96 1 y-Proteobacteria (Phylum XII) Oceanospirillales Alkalimonas collagenimarina (AB270706) 94-95 5 Oceanospirillales Halomonas aquamarina (AJ306891) 97 3 Oceanospirillales Marinomonas ostreistagni (AB242868) 91-92 3 Oceanospirillales Marinospirillum alkaliphilum (AF275 713) 92-98 12 3 Oceanospirillales Nitrincola lacisaponensis (AY567473) 98 8 Chromatiales Alkalilimnicola halodurans (AJ404972) 87 2 Chromatiales Ectothiorhodospira shaposhnikovii (M59 151) 99 7 Chromatiales Thiorhodospira sibirica (AJ006530) 97 1 8-Proteobacteria (Phylum XII) B de llo vibrio nales Bacteriovorax marinus (AF084854) 89 1 Desulfobacterales Desulfobotulus sapovorans (M34402) 95 1 Desulfovibrionales Desulfonatronum lacustrae (AF4 18171) 98 2 Desulfuromonadales Geobacter grbiciae (AF335182) 86 1 Firmicutes (Phylum XIII) Bacillales Bacillus agaradhaerens (X76445) 95 4 Bacillales Bacillus subtilis (DQ207730) 98-99 7 Bacillales Gracilibacillus dipsosauri (X82436) 97 3 Clostridiales Anoxynatronum sibiricum (AF522323) 94 1 Clostridiales Clostridium papyrosolvens (X71852) 86 1 Clostridiales Fusibacterpaucivorans (AF050099) 90 1 Clostridiales Heliorestis daurensis (AF079102) 96 2 Thermoanaerobacterales Moorella glycerini (U82327) 84 1 Actinobacteria (Phylum XIV) Acidimicrobiales Ferrimicrobium acidiphilum (AF251436) 79 1 Thermoleophilales Thermoleophilum album (AJ458462) 75 2 Spirochaetes (Phylum XVII) Spirochaetales Leptospira interrogans (AY631894) 76 1 Spirochaetales Spirochaeta alkalica (X93927) 95 2 Spirochaetales Spirochaeta asiatica (X93926) 91 3 Spirochaetales Spirochaeta aurantia (M57740) 87 2 Spirochaetales Spirochaeta stenostrepta (M88724) 90 1 Fibrobacteres (Phylum XVIII) Fibrobacterales Fibrobacter succinogenes (M62689) 79 1 Bacteroidetes (Phylum XX) Sphingobacteriales Aquiflexum balticum (AJ744861) 93-95 8 Sphingobacteriales Belliella baltica (AJ564642) 90 1 1 Sphingobacteriales Cyclobacterium lianum (DQ534063) 87-88 4 1 Sphingobacteriales Aquirestris calciphila (AJ786341) 88 1 Sphingobacteriales Fulvivirga kasyanovi (DQ836305) 85-87 7 Sphingobacteriales Gracilimonas tropica (EF988655) 84 1 Flavobacteriales Sediminibacterfurfurosus (AB255369) 85 1 Lentisphaerae Victivallis vadensis (AY049713) 76 3 Tenericutes Acholeplasmatales Acholeplasma axanthum (AF4 12968) 8! 1 Somogyi, B., Vanyovszki, J., Agyi, A., Vörös, L. (2007) Eukarióta és prokarióta pikoalga törzsek fotoszintézisének összehasonlító vizsgálata. Hidrol. Közi. 87 (6): 119Vörös, L., V-Balogh, K., Boros, E. (2005) Pikoplankton dominancia szikes tavakban. Hid. Közi. 85 (6): 166-168. Yakimov, M. M., Giuliano, L., Chernikova, T. N., Gentile, G., Abraham, W. R., Lunsdorf, H., Timmis, K. N., Golyshin, P. N. (2001) Alkalilimnicola halodurans gen. nov., sp. nov., an alkaliphilic, moderately halophilic and extremely halotolerant bacterium, isolated from sediments of soda-depositing Lake Natron, East Africa Rift Valley. Int. J. Syst. Evol. Microbiol. 51. 2133-2143. Zahr, M., Fobel, B., Mayer, H., Imhoff, J. F., Campos, V.,Weckesser, J. (1992) Chemical composition of the lipopolysaccharides of Ectothiorhodospira shaposhnikovii, Ectothiorhodospira mobilis, and Ectothiorhodospira halophila. Arch. Microbiol. 157:499-504. Zhang, W., Xue, Y, Ma, Y, Grant, W. D., Ventosa, A., Zhou, P. (2002) Marinospirillum alkaliphilum sp. nov., a new alkaliphilic helical bacterium from Haoji soda lake in Inner Mongolia Autonomous Region of China. Extremophiles 6: 33-37. Zhilina, T. N., Zavarzin, G. A., Rainey, F., Kevbrin, V. V, Kostrikina, N. A., Lysenko, A. M. (1996) Spirochaeta alkalica sp. nov., Spirochaeta africana sp. nov., and Spirochaeta asiatica sp. nov., alkaliphilic anaerobes from the Continental Soda Lakes in Central Asia and the East African Rift. Int. J. Syst. Bacteriol. 46: 305-312. Yakimov, M. M., Giuliano, L., Chernikova, T. N., Gentile, G., Abraham, W. R., Lunsdorf, H., Timmis, K. N„ Golyshin, P. N. (2001) Alkalilimnicola halodurans gen. nov., sp. nov., an alkaliphilic, moderately halophilic and extremely halotolerant bacterium, isolated from sediments of soda-depositing Lake Natron, East Africa Rift Valley. Int. J. Syst. Evol. Microbiol. 51. 2133-2143. Zahr, M., Fobel, B., Mayer, H., Imhoff, J. F., Campos, V.,Weckesser, J. (1992) Chemical composition of the lipopolysaccharides of Ectothiorhodospira shaposhnikovii, Ectothiorhodospira mobilis , and Ectothiorhodospira halophila. Arch. Microbiol. 157:499-504. Zhang, W., Xue, Y, Ma, Y, Grant, W. D., Ventosa, A., Zhou, P. (2002) Marinospirillum alkaliphilum sp. nov., a new alkaliphilic helical bacterium from Haoji soda lake in Inner Mongolia Autonomous Region of China. Extremophiles 6: 33-37. Zhilina, T. N., Zavarzin, G. A., Rainey, F., Kevbrin, V. V, Kostrikina, N. A., Lysenko, A. M. (1996) Spirochaeta alkalica sp. nov., Spirochaeta africana sp. nov., and Spirochaeta asiatica sp. nov., alkaliphilic anaerobes from the Continental Soda Lakes in Central Asia and the East African Rift. Int. J. Syst. Bacteriol. 46: 305-312. Diversity investigations on planktonic bacterial communities of Böddi-szék (Kiskunság) during an algal bloom Borsodi A., Knáb M., Czeibert K., Palatinszky M., Kreit G., Somogyi B., Vörös L., Márialigeti K. Abstract: Phototrophic microorganisms, mainly pikocianobacteria are dominant in the alkaline and salt water of the extremely shallow soda ponds located in Kiskunság National Park. The aim of the present study was to reveal the bacterial diversity of the water of Böddi-szék during an early summer algal bloom. Water samples were spread onto different media and 93 bacterial strains were isolated. Following ARDRA grouping the 16S rRNA gene of 66 representatives was sequenced. Most of the strains belonged to alkaliphilic or alkalitolerant and moderately halophilic species of genera Bacillus, Gracilibacillus, Algoriphagus, Aquiflexum, Alkalimonas, Nitrincola and Halomonas. Other strains were closely related to alkaliphilic and phototrophic purple non sulfur bacteria (Erythrobacter és Rhodobaca). Analysis of the clone library revealed the dominance of Proteobacteria, Bacteroidetes és Spirochaetes. The results of this study suggest the presence of a complex microbiota based on the primary production of photosynthetic microorganisms such as the detected Prochlorococcus, Anabaena, Rhodobaca, Rhodobacter, Rhodovulum, Ectothiorhodospira. On the other hand, microbes with chemo-heterotrophic metabolism are able to decompose organic matters aerobically (Bacillus , Halomonas, Alkalimonas ) or anaerobically ( Desulfonatronum , Geobacter, Desulfobotulus, Anoxynatronum, Spirochaeta).
