Hidrológiai Közlöny 2009 (89. évfolyam)

6. szám - L. Hidrológiai Napok: "A hazai hidrobiológia ötven éve" Tihany, 2008. október 1-3.

195 6. ábra Moina brachiata egyedek földrajzi régiónként való felosztása, (CVA, SYN-TAX). • - Hortobágyról, • - Körös-Maros Nemzeti Parkból, •- Kiskunságból. Vektorok: 1 - tollas sörte, 2 - fésű, 3 - aránypár Irodalom Bjerke, J. M„ Freeman, T. P. & Anderson, A. W., 1974. A new method of preparing insects for scanning electron microscopy. Entomology Department and Botany Department, North Dakota State University, Fargo, North Dakota, Stain Technology Forró, L„ 2005. What is Moina brachiata (Jurine 1820) - an attempt to deftne it based on morphological features. Abstract Book, Vllth International Symposium on Cladocera Goulden, C. E., 1968. The systematics and evolution of the Moinidae. Transactions of the American Philosophical Society 58: 1-101 Gulyás, P. & Forró, L., 1999. Az ágascsápú rákok (Cladocera) kishatározója 2.(bővített kiadás), Vízi Természet- és Környezetv. Budapest Muller, L. L. & Jacks T. J„ 1974. Rapid chemical dehydration of samples for electron microscopic examinations. Journal of Histochemistry and Cytochemistry 23:107-110 Nédli, J„ Major, A. & Forró, L., 2007. Increase in biodiversity due to adaptation to different ecological conditions in temporary pools. lst Euró Diversity Conference, Abstract Book p. 33. Fine morphological research on moina brachiata N. Flórián, J. Nédli Abstract: Moina brachiata (Jurine, 1820) belonging to Cladocera, is a typical species occuring in temporary waterbodies in the Great Hungárián Plain. Enzyme polymorphism investigations of the species in Hungary recently showed significant genetic differentiation of the populations. It is likely that two different lineages exist within M. brachiata, one of them linked to habitats with high salinity whilst the other to lower salinity. Despite of the stricking differentiation appearing in genetic markers, the light microscopy based morphometrical surveys so far could not detect different groups within the species (Forró 2005 and Nédli et al. 2007). In our survey we tried to find morphological characters that could help to separate the two different lineages. We used scanning electron microscopy to provide higher resolution than light microscope techniques. In the first place we scanned the characters that are used in identification keys and are unique to this species: the number of the large spines (comb) at the base of the terminal-claw, the ratio of the size and density of small spines on the distal part of the terminal-claw, the number of the feathered spines on the post-abdomen.Throughout the research we examined the post-abdomen of 60 specimens belonging to populations occurring in temporary waters of different salinity. After the multi-variate analysis of the data (hierarchical clustering, principal coordinate analysis), notable separation within Moina brachiata could not be detected on the basis of the characters mentioned above. Slight separation was discovered by principal component analysis in connection to the number of the feathered spines. Investigations of populations from further sampling sites are necessary to draw unambiguous conclusions on the morphology based separation of the Moina brachiata lineages. Keywords -.Moina brachiata, Crustacea, scanning electron microscope, morphology Podani, J„ 2001. SYN-TAX 2000. Computer programs for data analysis in ecology and systematics. User's manual. Scientia Publishing, Budapest 8. ábra, Utópotroh (a. tollas sörte, b. fésű, c. végkarmon lévő apróbb fogak) 7. abra Moina brachiata habituskepe, felig „behúzott utópotrohhal (a. utópotroh, b. első csáp, c. fei, d. héiszeeélv)