Hidrológiai Közlöny 2007 (87. évfolyam)

6. szám - XLVIII. Hidrobiológus Napok: Európai elvárások és a hazai hidrobiológia Tihany, 2006. október 4–6.

44 HIDROLÓGIAI KÖZLÖNY 2007. 87. ÉVF. 6. SZ. tett, ill. más minősítési rendszerek része lehet a különböző enzimek alapján is megtörténő ökológiai jellegű vízminősítés. Köszönetnyilvánítás A tanulmány az Országos Tudományos Kutatási Alap (K 60452), az Öveges József Program (HEF 06 01; Dinoalga), Bolyai János Kutatási Ösztöndíj és a Kutatás-fejlesztési Pályázati és Kutatáshasznosítási Iroda (PL-9/2005) támogatásával készült. Külön köszönjük a kézirat bírálójának rendkívül hasznos javaslatait. Irodalom Aaronson, S. and Patni, N. J. (1976) The role of surface and extracellular phosphatases in the phosphorus requirement of Oochromonas. Limnol. Oceanogr. 21, 838-845. Berman, T. (1970) Alkaline phosphatases and phosphorus availability in Lake Kinneret. Limnol. Oceanogr. 15, 663-674. Boavida, M. J.- Heath, R. T. (1984) Are the phosphatases released by Daphnia magna components of its prey? Limnol. Oceanogr. 29, 641-645. Buchanan, E. L. (1982) Planktonic photoheterotrophic and heterotrophic uptake, glucose in two acid lakes and one eutrophic lake in northeastern Ohio. Ph.D. thesis. Kent State University, Kent, Ohio. Dedysh S. N. (2002): Methanotrophic Bacteria of Acidic Sphagnum Peat Bogs. Microbiology. 71. (6). 638-650 Dévai, Gy., Dévai I., Felföldy L., Wittner I. (1992a) A vízminőség fogalomrendszerének átfogó koncepciója. 3. rész: Az ökológiai vízminőség jellemzésének lehetőségei. Acta biol. debrecina. Suppl. Oecol. Hung. 4: 49-185. Dévai, Gy., Juhász-Nagy P., Dévai I. (1992b) A vízminőség fogalomrendszerének átfogó koncepciója 2. rész.: A hidrobiológia és a biológiai vízminőség fogalomkörének értelemzése. Acta. biol. Debrecina, Suppl. oecol. hung. 4: 29-47. Dévai Gy., Végvári P., Nagy S., Bancsi I., Müller Z., Csabai Z„ Bárdosi E., Gőri Sz., Grigorszky I., Győriné M. B., Juhász P., Kaszáné K. M., Kelemenné Sz. E., Kiss B., Kovács P., Macalik K, Móra A., Olajos P., Piskolczi M., Teszárné N. M., Tóth A., Turcsányi I., Zsuga K., 1999: A Boroszló-kerti-Holt-Tisza ökológiai vízminősége. Acta Biol. Debr. Oecol. Hung. 10/1. Faust, M. A. and Gantt, E. (1973) Effect of light intensity and glycerol on the growth, pigment composition, and ultrastructure of Chroomonas sp. J. Phycol. 9,489-495. Faust, M. A., Sager, J. C., and Meeson, B. W. (1982) Response of Prorocentrum mariae-lebouriae (Dinophyceae) to light of different spectral qualities and irradiance growth and pigmentation. J. Phycol. 18, 349-356. Fitzgerald, G. P.- Nelson, T. C. (1966) Extractive and enzymatic analyses for limiting or surplus phosphorus in algae. J. Phycol. 2, 32-37. Francko, D. A. and Heath, R. T. (1979) Functionally distinct classes of complex phosphorus compounds in lake water. Limnol. Oceanogr. 24, 463-473. Francko, D. A. and Heath, R. T. (1983) Abiotic uptake and photodependent release of phosphate from high molecular weight humic-iron complexes in bog lakes, p. 467-480 In R. F. Christman and E. T. Gjessing [eds.], Aquatic and Terrestrial Humic Materials. Ann. Arb, Science, Ann. Arbor, MI. Hanes, C. S. (1932) The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley. Biochem. J. 26, 1406-1421. Jansson, M., Olsson, H. and Broberg, O. (1981) Characterization of acid phosphatases in the acidified Lake Gardsjon, Sweden. Arch. Hydrobiol. 92, 377-395. Kopf,V.W. (1983) Untersuchungen zur Dynamik der Chlorophyll-Konzentration in natürlichen Phytoplanktonpopulationen. Arch. Hydrobiol. 98, 173-180. Kuenzler, E. J. (1970) Dissolved organic phosphorus excretion by marine phytoplankton. J. Phycol. 6, 7-13. Kuenzler, E. J. and Perras, J. P. (1965) Phosphatases of marine algae. Biol. Bull. 128, 271-284. Lehman, J. T. (1980) Cycling of P among phytoplankton, herbivorous Zooplankton and the lakewater, In D. Scavia and R. Moll [eds.], Nutrient Cycling in the Great Lakes: A Summarization of Factors Regulating the Cyeling of Phosphorus. Spec. Rept. No. 83, Great Lakes Res. Div., Univ. of Michigan, Ann Arbor, MI. Murphy, J. and Riley, J. P. (1962) A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta 27,31-36. Prescott, G. W. (1962) Algae of the Western Great Lakes Area. 977 pp. W.C. Brown Co., Dubuque, Iowa Reichardt, W„ Overbeck, J. and Steubing, L. (1967) Free dissolved enzymes in lake waters. Nature 216, 1345-1347. Stevens, R. J. and Parr, M. (1977) The significance of alkaline phosphatase activity in Lough Neagh. Freshwat. Biol. 7, 351-355. Stewart, A. J. and Wetzel, R. G. (1982) Influence of dissolved humic materials on carbon assimilation and alkaline phosphatase activity in natural algal-bacterial assemblages. Freshwat. Biol. 12, 369-380. Strickland, J. D. H. and Parsons, T. R. (1968) A Practical Handbook of Seawater Anal. Bull. Fish. Res. Bd. Can. Ottawa, Canada, 293 pp. USEPA (1971) Methods for Chemical Analysis of Water and Wastes. National Env. Res. Ctr., Cincinnati, OH. Wetzel, R. G. (1981) Longterm dissolved and particulate alkaline phosphatase activity in a hardwater lake in relation to lake stability and phosphorus enrichments. Verh. Intern. Verein. Limn. 21, 369-381. Wetzel, R. G. (1983) Limnology. 2nd Edition. Saunders College Publishing, Philadelphia, PA, 858 pp. Importance of acidic phosphatase activity in peat bogs Grigorszky, I. - Borics, G. 2. Béres, V. 3 - Farkas, T.

4 'Department of Botany, University of Debrecen, H-4010. Debrecen, P. O. Box 71, Hungary Environmental Protection Inspectorate for Trans-Tiszanian Region, H-4025. Debrecen, Piac utca 9/b, Hungary 'Department of Botany, University of Debrecen, H-4010. Debrecen, P. O. Box 14, Hungary 4AggteIek National Park H-3758. Jósvafő Tengerszem oldal 1. Abstract: Phosphomonoesters are capable of releasing phosphate through cleavage of phosphoester bonds by phosphatase enzymes. The seasonal importance of this process was examined by using a model substrate paranitrophenylphosphate and the Michaelis-Menten equation to estimate the release rate of P0

4-P from phosphomonoesters. The seasonal occurrence of phosphomonoesters and acid phosphatase activity was used to estimate the velocity of phosphate release from these compounds. Filter fractionation of phosphatase activity demonstrated most activity (> 60%) was in size fractions less than 0.45 um. The release rates were highest in May (15 nmol L"' min "') and also in June (25 nmol L"

1 min ) during the Gonyostomum semen (Raphidophyta) bloom but decreased to less than 2 nmol L 1 min"

1 within in two weeks and remained at this level or lower throughout the summer and the fall. Fractionation of 3

2P-H3P04 labelled dissolved organic phosphorus showed this fraction to vary considerably through the year. Potential phosphate release declined through the summer and into the fall. Significance of the co-occurrence of phosphomonoesters and acid phosphatase activity maxima is discussed.