Hidrológiai Közlöny 2003 (83. évfolyam)

3. szám - Ördög Vince–Balogi Zsolt: Cylindrospermopsis raciborski bioteszt: mederüledék minták biológiailag hozzáférhető foszfor koncentrációjának a mérésére

ÖRDÖG V - BALOG! 7.S.: CylirKlrospermopsis raciborskii bioteszt 167 Huettl, P. J., R. C. Wendí and R. B. Corey. 1979. Prediction of algal-available phosph in runoff suspensions. J. Environ. Qual. 8 :130-132. ISO 10260. 1993. Vízminőség A biokémiai paraméterek mérése. Az aklorofill-koncentráció spektrofotometriás meghatározása. Magyar Szabványügyi Hivatal, Budapest Kleeberg, A. and J.-G. Kohl. 1999. Assessment of the long-term effectiveness of sediment dredging to reduce benthic phosphorus release in shallow Lake Müggelsee (Germany) Hydrobiologia 394:153-161 Marvan, P., S. Pribil and O. Lhotsky (ed.). 1979 Algal assays and monitoring eutrophication. Schweizerbart'sche Verl.buchhandl.. Stuttgart Miller, W.E., J.C. Greene and T. Shiroyama. 1978. The Selenastrum capricomulum Printz algal assay bottle test. Experimental design, application and data interpretation protocol. EPA-600/9-78-0

18. U.S. Environmental Protection Agency, Corvallis, Ore. Murphy, J. and J.P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters Anal. Chim. Acta 27:31-36. Noges, P. and A. Kisand. 1999. Fonns and mobility of sediment phosphorus in shallow eutrophic Lake Vortsjarv (Estonia). Internat. Rev. Hydrobiol. 84:255-270 Nyholm, N. and J.E. Lyngby. 1988. Algal bioassays in eutrophication research - a discussion in the framework of a mathematical analysis. Wat Res 22:1293-1300. Ogilvie, B.G. and S.F. Mitchell. 1998 Does sediment resuspension have persistent effects on phytoplankton? Experimental studies in three shallow lakes. Freshwat. Biol. 40:51-63. Ördög V. 1981a. Contributions to the standardization of laboratory alga tests. Bot. Közlem. 68:85-93. Ördög V. 1981b Apparatus for laboratory algal bioassay. Int. Revue ges. Hydrobiol. 67:127-136. Ördög V. and Dobolyi. F.. 1997. Estimation of BAP in the sediment of L Balaton by chemical fractionation and bioassay Hidr Közi. 77:5-6. Ördög V. and Máté F. 2001. Algal bioassay for determination of phosphorus availability. Agrokémia és Talajtan (in Press) Persson, P. 1990. Utilization of phosphorus in suspended particulate matter as tested by algal bioassays Verh Internat. Verem. Limnol 24:242-246. Rippka, R., T. Deruelles, T.B. Waterbury, M. Herdman and R.J. Stanier 1979. Genetic assignment, strain histories and properties of pure cultures of cyanobacteria. J Gen. Microbiol

. 111:1-61. Rossi, G and G. Prema/A. 1991. Delay in lake recovery caused by internal loading. Wat. Res. 5:567-575. Ruban, V. and D. Demare. 1998. Sediment phosphorus and internal phosphate flux in the hydroelectric reservoir of Bort-Ies-Orgues, France Hydrobiologia 373:349-359. Schreiber, E. 1927 Die Reinkultur von marinen Phytoplankton und deren Bedeutung für die Erforschung der Produktionsfähigkeit des Meerwassers. Wissenschaftliche Meeresuntersuchungen aus der Biologischen Anstalt auf Helgoland, Kiel Sagher, A., R. Harris and D A. Armstrong. 1975. Availability of sediment phosphorus to microorganisms Report WRC 75-01. Water Resource Center, University of Wisconsin, Madison, Wl. Sharpley, A.N. 1993. Estimating phosphorus in agricultural runoff available to several algae using iron-oxide paper strips. J. Environ. Qual. 22:678-680. Skulberg, O. 1964. Algal problems related to the eutrophication of European water supplies, and a bio-assay method to assess fertilizing influences of pollution on inland waters, p 262-299. In: D.F. Jackson (ed.) Algae and man. Plenum Press, New York. Staub, R. 1961. Emährungsphysiologisch-autökologische Untersuchungen an der planktischen Blaualge Oscillaloria rubescens DC. Schweizerische Z. für Hydrol. 23:82-198. A kézirat beérkezett: 2003. február 26. ÖRDÖÍÍ VINCE okleveles agrármérnök, a biológiai tudomány kandidátusa, az MTA Hidrobiológiái Bizottságának és Növényélettani Bizottságának tagja. A Nyugat-Magyarországi Egyetem Mezőgazdaság- és Élelmiszer-tudományi Karának dékánja, a Növényélettan és Növényi Biotechnológia Tanszék vezetője. Négy és fél évig hidrobiológus szakértőként dolgozott Brazíliában. Szűkebb szakterülete a halastavak hidrobiológiája, valamint a mikroalgák élettana és biotechnológiája. BALOG! ZSOL T okleveles vegyész (Eötvös Loránd Tudományegyetem, 1985), műszeres analitikai szakmérnök (Veszprémi Egyetem). A Közép-Dunántúli Környezetvédelmi Felügyelőség mérőállomás vezetője 1991. ótaCylindrospermopsis raciborskii biotest for the determination of the BAP concentration of sediment samples Ördög, V. - Balogi, Zs. Abstract: The algal assay bottle test, using the green alga Raphidocetis subcapitata (Kors.) Nyg. for the determination of the algal available phosphorus forms (BAP), has been widely used for more than two decades. The question is whether the resulLs of a green algal test are sufficient for the reliable determination of BAP, or not. To answer this question we determined the BAP value in sediment samples of the six Hungarian shallow standing water bodies In these tests the cyanobacterium Cvlindrospermopsis raciborskii (Wol.) Seenayya et Subba Raju was also used, in addition to R. subcapitata The green alga was invesligated in Z-8 nutrient solution, while the cyanobacterium was tested in N-free and also in N-containing BO-1

1 nutrient solutions. The highest BAP concentrations were measured when using C. raciborskii in N-free BG11 nutrient solution. The average BAP concentration of these samples was 128 fig.g"

1, while the minimum and maximum values were 33 and 717 ng.g'

1, respectively. For C. raciborskii in N-containing BG-11 nutrient solution and R. subcapitata in Z-8 nutnent solution the average BAP values measured were 66 and 69 fig.g ', respectively. The respective minimum and maximum values were 6 és 593 ng.g ', and 4 ^ig.g'

1 and 596 |ig.g"'. The fluctuation of the ratio of the BAP values measured with the two test organisms increased with the decrease of BAP. The results indicate that the biotest for the determination of the BAP concentration of sediment samples should be cany out by both the green alga R. subcapitata. and by the cyanobacterium C. raciborskii grown in N-free BG-11 nutrient solution. Key words: Hydrobiologics, alga, biotest.