Fogorvosi szemle, 2014 (107. évfolyam, 1-4. szám)
2014-06-01 / 2. szám
49 FOGORVOSI SZEMLE ■ 107. évf. 2. sz. 2014. 20. Kuehnle I, Goodell MA: The therapeutic potential of stem cells from adults. BMJ 2002; 325: 372-6. 21. Kyrylkova K, Kyryachenko S, Biehs B, Klein O, Kioussi C, Leid M: Bell 1b regulates epithelial proliferation and asymmetric development of the mouse mandibular incisor. PLoS One 2012; 7: e37670. 22. Laino G, d’Aquino R, Graziano A, Lanza V, Carinci F, Naro F és mtsai: A new population of human adult dental pulp stem cells: A useful source of living autologous fibrous bone tissue (lab). J Bone Miner Res 2005; 20: 1394-402. 23. Le Blanc K, Pittenger M: Mesenchymal stem cells: Progress toward promise. Cytotherapy 2005; 7: 36-45. 24. Ma J, Wang Y, Yang J, Yang M, Chang KA, Zhang L és mtsai: Treatment of hypoxic-ischemic encephalopathy in mouse by transplantation of embryonic stem cell-derived cells. Neurochem Int 2007; 51: 57-65. 25. Michalczyk K, Ziman M: Nestin structure and predicted function in cellular cytoskeletal organisation. Histol Histopathol 2005; 20: 665-71. 26. M iura M, G ronthos S, Z hao M, Lu B, Fisher LW, R obey PG és mtsai: Shed: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sei USA 2003; 100: 5807-12. 27. Molnár B, Kadar K, Király M, Porcsalmy B, Somogyi E, Hermann P és mtsai: [isolation, cultivation and characterisation of stem cells in human periodontal ligament]. Fogorv Sz 2008; 101: 155-61. 28. Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 1997; 276: 71-4. 29. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J és mtsai: Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004; 364: 149-55. 30. Tucker A, Sharpe P: The cutting-edge of mammalian development; how the embryo makes teeth. Nat Rev Genet 2004; 5: 499-508. 31. Vemuri MC, Chase LG, Rao MS: Mesenchymal stem cell assays and applications. Methods Mol Biol 2011 ; 698: 3-8. 32. Vollner F, Ernst W, Driemel O, Morsczeck C: A two-step strategy for neuronal differentiation in vitro of human dental follicle cells. Differentiation 2009; 77: 433-41. 33. Yu J, He H, Tang C, Zhang G, Li Y, Wang R és mtsai: Differentiation potential of stro-1+ dental pulp stem cells changes during cell passaging. BMC Cell Biot, 11: 32. Kálló K, Gánti B, Kerémi B, Stiedl P, Nagy Á, Varga G, Nagy K. Cultivation and Differentiation of Rat Dental Pulp Stem Cells for Tissue Engineering Purposes Although existence of dental stem cells has been known since 2000, and these are already well characterized, hardly any data are available regarding rat dental pulp cells. The aim of our study was to isolate, cultivate and differentiate primary cell cultures, showing stem cell properties, from rat incisor pulp. Cell viability was determined using the WST-1 test. Osteogenic differentiation potential was examined by von Kossá and Alizarin Red staining, and the neurogenic potential by morphological studies, immunocytochemistry and quantitative PCR. Moreover, the effect of different surface coatings on neurogenic differentiation was also investigated. We have succeeded in isolating stem cell-containing cell cultures from the pulp tissue of rat lower incisors and optimized the culture conditions. We have provided evidence that these cell cultures are capable for differentiating towards both osteogenic and neurogenic lineages. We have developed a novel neurogenic differentiation protocol, which is more effective than the previous one. Lysine-laminin and ornithinlaminin coated surfaces were found to be better for rat pulp-cell differentiation. Our results confirm that dental-pulp stem cells are capable of regenerating injured bone and neural tissue. Our model provides the foundation for the clinical use of dental pulp stem cells for tissue engineering purposes. Key words: rat, pulp, stem cell, osteogenic, neurogenic, differentiation, culture, tissue engineering
