Fogorvosi szemle, 2011 (104. évfolyam, 1-4. szám)
2011-03-01 / 1. szám
18 FOGORVOSI SZEMLE ■ 104. évf. 1. sz. 2011. gical treatment of experimental peri-implantitis with autogenous bone graft and ePTFE membrane in cynomolgus monkeys. Clin Oral Impl Res 2003; 14: 412-422. 35. Schou S, Holmstrup P, Jorgensen T, Stoltze K, Hjorting-Hansen E, Wenzel A: Autogenous bone graft and ePTFE membrane in the treatment of peri-implantitis. I. Clinical and radiographic observation in cynomolgus monkeys. Clin Oral Impl Res 2003; 14: 391-403. 36. Schwarz F, Bieling K, Bonsmann M: Nonsurgical treatment of moderate and advanced periimplantitis lesions: a controlled clinical study. Clin Oral Invest 2006; 10: 279-288. 37. Stájer A, Ungvári K, Pelsőczi KI, Polyánka H, Oszkó A, Mihalik E, Rakonczay Z, Radnai M, Kemény L, Fazekas A, Turzó K: Corrosive effects of fluoride on titanium: investigation by X-ray photoelectron spectroscopy, atomic force microscopy, and human epithelial cell culturing. J Biomed Mater Res A 2008; 87: 450-458. 38. Uribe R, Penarrocha M, Sanchis JM, Garcia O: Marginal peri-implantitis due to occlusal overload. A case report. Med Oral 2004; 9: 159-162. 39. Zitzmann NU, Berglundh T: Definition and prevalence of peri-implant diseases. Review. J Clin Periodontol 2008; 35 {Suppl.8): 286- 291. Dr. Ungvári K, Dr. Pelsőczi KI, Kormos B, Oszkó A, Prof. Dr. Rakonczay Z, Dr. Radnai M, Prof. Dr. Kemény L, Prof. Dr. Nagy K, Prof. Dr. Fazekas A, Dr. Turzó K: Impact of decontaminating solutions on titanium surface: an epithelial cell culture study Introduction The effects of three different decontaminating solutions in clinical use for peri-implantitis therapy on the chemical structure and surface roughness of commercially pure (CP) Ti were investigated. A further aim was to survey the response of the biological environment to these changes, by examining the attachment and proliferation of human epithelial cells after treatment of the Ti surfaces with these solutions. Materials and methods CP (grade 4) machined titanium discs (CAMLOGTM Biotechnologies AG, Switzerland) were treated with 3% H202 (5 min), saturated citric acid (pH = 1; 1 min) or chlorhexidine gel (CHX, 5 min). The surface properties were followed through the use of X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The epithelial cell attachment and proliferation was examined by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and bicinchoninic acid (BCA) protein-content assays. Results XPS showed an intact Ti02 layer on each sample and CHX was adsorbed by the surface, as C-O and/or C=0 bond formation was revealed. AFM results gave no significant changes in the roughness after treating the surfaces with the cleaning solutions. While MTT and BCA assays did not show significant differences in epithelial cell attachments, the cell proliferation was significantly increased after H202 treatment as compared to CHX (not shown by BCA assays). Conclusions The applied decontaminating agents do not damage the Ti surface. H202 can be used effectively in decontaminating the implants affected by peri-implantitis, as the human epithelial cell growth was improved, in contrast with CHX. Key words: peri-implantitis, implant surface, decontamination, epithelial cell culture PÁLYÁZAT KÖRMÖCZI-PÁLYADÍJRA Felhívjuk minden, a Fogorvosi Szemlében publikáló, 35 évnél fiatalabb első szerzős cikk szerzőjét, hogy pályázzanak a 2010-es Körmöczi-pályadíjra. Pályázni csak a 2010-ben, a Fogorvosi Szemlében megjelent közleményekkel lehet. Kérjük, a közlemény különlenyomatának egy példányát mellékelje a pályázathoz. A pályázat beadási határideje: 2011. július 15. A pályázatokat, kérem, postán juttassák el a címemre. Dr. Tóth Zsuzsanna az MFE főtitkára SE Konzerváló Fogászati Klinika 1088 Budapest, Szentkirályi utca 47.
