Fogorvosi szemle, 2005 (98. évfolyam, 1-6. szám)
2005-04-01 / 2. szám
80 FOGORVOSI SZEMLE ■ 98. évf. 2. sz. 2005. microscope and focused via the objective lenses to a micron-sized spot diameter. Within the narrow laser focal spot forces are generated that allow ablation of material (that is cutting; Laser Micro-dissection), whilst the surrounding tissue remains fully intact. Using the same laser the separated cell(s) or selected tissue areas can be lifted up (Laser Pressure Catapulting) and captured in a collection device. This is a totally non-contact process, as only focused light is used for the transportation of a selected area into a collection device. Targets sized from parts of chromosomes up to an entire living organism, as the nematode C. elegans, are successfully transported without impairing biological information or viability of the specimen. Besides that, the focused laser allows to poke minute holes into cells, which are closed by the cell itself within a few seconds or minutes. This enables injection of, e.g., drugs or genetic material without using viral vectors or chemical treatment of the cells. The method of laser mediated live cell handling promises to take a big step forward in all fields of science related to the study of live cells. - www.palm-microlaser. com -Keywords: Laser Microdissection, live cells, microscope A. SKAGERS, K. STAMERS, L. FELDMANE, L. BERZINA*, R. CIMDINS* Riga Stradins University, Latvia; * Riga Technical University RESPONSE OF RABBIT EAR CARTILAGE TO IMPLANTATION OF CALCIUM PHOSPHATE BIOCERAMICS Looking for scaffold material to provide bioengineering of cartilage tissue structures we performed experimental morphological study of reactogenicity of HA, TCP and HA/TCP (1:1) granules implanted in rabbit ear supraperichondraly , subperichondraly and in defect after excision of cartilage. Samples were taken out after 4 and 8 weeks, fixed in 10 % neutral formalin, embedded in paraffin, stained using hematoxylin - eozin, combinated van Gieson and Romeis methods, PAS reaction combined with alcian blue (pH 2,5). After 4 weeks in surrounding soft tissue is small amount of inflammatory cells only vascular response and swelling in intercellular space. Differentiation and proliferation of large chondroblastic cells are observed more in cases of excisional cartilage wounds as after supra- or subperichondral implantation. After 8 weeks no inflammatory cells were obtained. There was no significant difference in tissue response on different porous ceramic materials. The lot of large chondroblastic cell proliferation like eruption of volcano with increase of acid glycosaminoglycans in new formed cartilage was observed in cartilage defect wounds filled with HA, TCP and HA/TCP granules. In surrounding old cartilage increase of neutral glycosamins was observed. In cases where excisional wound of ear cartilage was separated from surrounding soft tissue by HA blocks pure chondrogenic tissue regeneration occurred while without this separation mixed predominantly fibrous tissue proliferation in defect developed. Porous calcium phosphate bioceramic materials have good biocompatibility and chondroidinductive property implanted in rabbit ear. Similar tissue response but different solubility and biodegradation rate of HA, TCP and HA/TCP gives possibility for different longevity of scaffolds necessary for different design and function of bioengineered cartilage tissue structures. Keywords: cartilage, calcium phosphate ceramic Acknowledgement: Support of COST Action B23 is gratefully acknowledged. K. SUZUKI , Y. KOZAWA Department of Anatomy, Nihon University, School of Dentistry at Matsudo. 870-1, Sakae-cho Nishi 2, Matsudo, Chiba, 271-8587, Japan IDENTIFICATION OF DENTITION AND TOOTH REPLACEMENT OF EXTINCT MAMMALS.- IN CASE OF DESMOSTYLUS CHEEK TEETH (MAMMALIA: DESMOSTYLIA) Desmostylus is a fossil mammal that lived in the Miocene in the North Pacific region. The molar consists of columnar cusps with a large crater-like depression of each. The molars have been thought to be horizontal replacement. In our previous studies, the developmental process of the molar has been clarified by the incremental lines and proposed that Desmostylus had two molars in each jaw half. Thus the previous dental formula (0.1.3.3/1.1.3.3) should be changed to (0.1.3.2/1.1.3.2). Indeed, the Utanobori specimens (LGSJ-F7743, lll:GSJ-F7745) and the Togari specimen (NSM-PV5600) exhibited in each jaw half one premolar and two molars. However, the Keton specimen (UHR18466) is reported to have three molars. Based on our measurement of the teeth, the most mesial molar should be determined to be a deciduous molar. The diastema between premolar and molar has been observed in those specimens. Upper jaw of the Utanobori III specimen had a plate-like substance between premolar and molar. In our observation on roots of molars and deciduous molars in upper jaw, disto-buccal root and lingual root fused and bifurcated close to apex and the horizontal section of the root showed long oval feature. The substance was determined to be the rest of root of the deciduous molar. It is confirmed that Desmostylus had two molars in each jaw half and deciduous molars were vertical replacement, owing to reinvestigation the molars and the diastema between premolar and molar.
