Fogorvosi szemle, 2000 (93. évfolyam, 1-12. szám)
2000-03-01 / 3. szám
intracellular pH and calcium in the rabbit mesenteric small artery. J. Mol. Cell. Cariold. 28(8), 1715, 1996. — 12. Nyárasdy I.: Kompozicids tömőanyagok biológiai akceptábilitásának vizsgálati lehetőségei, különös tekintettel a pulpa vitálmikroszkópjára. Fogorv. Szle. 83, 247, 1974. - 13. Palasz, M. V., Gerzina, T. M., Hume, W. R.: Cytotoxic and quantitive evaluation of leachable components from orthodontic adhesives (abstract). J. Dent. Res. 73, 436, 1994. — 14. Pohto, M., Scheinin, A.: Microscopic observation on hving dental pulp. I. Method for intravital study of circulation in rat incisor pulp. Acta Ódont. Scand. 16, 303, 1958. — 15. Swerdlow, H., Stanley, HR.: Reaction of the human dental pulp to cavity preparation, Part II At 15000 rpm with an air-water spray. J. Prosthet. Dent. 9, 121, 1959. - 16. Taylor, A. C.: Microscopic observation of the living tooth pulp Science. Ill, 40, 1950. - 17. Uno, S., Finger, WJ.: Effects of acidic conditioners on dentine demineralization and dimension of hybrid layers. J. Dent. 24(3), 211,1996. Dr. Iványi, I., Kispélyi B., Lázár, A., dr. Balogh, Á., dr. Rosivall, L., dr. Nyárasdy, L: Vitalmicroscopic Examination of the Rat Dental Pulp Exposed to Etching Material Materials employed in “adhesive filling technique” often lack informative instructions concerning the depth of cavities which require lining. Etching-materials directly placed on the dentin can adversely influence the pulp leading to changes in local microcirculation which can be an early sign of the pathological changes in the pulp. Therefore, it is worth examining the effect of these materials on the diameters of pulpal vessels. In the present study we examined wether acid etching (testi: 15s and test2: 60s) alters the blood circulation of the pulp lower incisors, by applying it directly onto a thin layer of pulpal wall (40-50 pm) without any liner, and following the instructions provided by the manufacturer. Twenty male Sprague-Dawley rats (weighing 330±51 g) and the technique of vitalmicroscopy was used for this investigation. The left lower incisors of the rats were prepared for vitalmicroscopy. Changes in vessel diameter were recorded prior to and 5, 15, 30 and 60 minutes after investigated materials or saline (control) were administered on dentin. In control and testi group the vessel diameter was unchanged during the experiment. However, in group test2 an intense vasoconstriction was registered. There were significant (p>0.05, ANOVA) differences in diameter changes between the control group and those treated with acid etching for 60s. Our results suggest that extended etching time may affect the circulatory system in the dental pulp, when applied onto very thin layer of dentin. Key words: biocompatibility, vitalmicroscopy, acid-etching, pulp, circulation . 82
