B. Papp szerk.: Studia Botanica Hungarica 34. 2003 (Budapest, 2003)
Vasas, Gizella: Investigations on macrofungi production effected by fungistasis at Pilisszentkereszt (Hungary)
cesses of growth and development in a positive way and they have fungistatic effect on the competitor microfungi species also among natural conditions. Fungistatic processes of bacterial origin were detected several times under laboratory conditions (PARK and AGNIHOTRI 1969, HUBBARD et al. 1983, DIX and WEBSTER 1994, LOCSMÁNDI and VASAS 2001). However, a several year long investigation, aiming at the issue of biological mass production of macrofungi under bacterium infected otherwise natural conditions, has not been carried out. Our aim was to find out if there was a difference between the amount of fruit-bodies and the species composition in infected sampling plots compared to control areas. Bacillus subtilis was applied as infecting agent, because it was easy to keep it in cultures having moderate requirements. MATERIAL AND METHODS Our investigation was started on 22 May 2000 in a mixed deciduous forest (dominated by Fagus, Carpinus and Quercus) at the right side of the road leading from Pilisszentkereszt to Pilisszántó. Two quadrates of 15 m x 15m were selected and marked. One of them was sprayed by 20 litre Bacillus subtilis cultures of 10 f ' cells/ml concentration monthly from May to November, while the other quadrate served as a control. In 2001, further two quadrates (treated and control) were selected in the nearby spruce forest. The sampling sites were visited 6 times during the year 2000, 7 times in 2001, and 8 times in 2002. All fruit-bodies were collected in both the treated and control quadrates, then the bacterium cultures were sprayed at the treated sites. Attributes of fungal coenology - such as variation of species composition and abundance compared to control - were investigated in 21 occasions within 3 years on 4 sampling sites. RESULTS The results of the fungal production in treated and control sites of the mixed forest (Table 1 ), and in treated and control sites from the spruce forest (Table 2) are summarised in Table 3. At the sampling site in the mixed deciduous forest (treated by Bacillus subtilis) 31 terricolous saprotrophic (S) species with 952 fruit-bodies, 15 lignicolous wood decaying (X) species with 279 fruit-bodies, 16 mycorrhizal (M) species with 163 fruit-bodies were detected. At the control site in the mixed forest 23 terricolous saprotrophic (S) species with 201 fruit-bodies, 7 lignicolous wood decaying (X) species with 74 fruitbodies, 17 mycorrhizal (M) species with 177 fruit-bodies were recorded. At the sampling site in the spruce forest (treated by Bacillus subtilis) 23 terricolous saprotrophic (S) species with 784 fruit-bodies, 209 of this is fruit-body
